Quantification of HIV-RNA from dried blood spots using the siemens VERSANT® HIV-1 RNA (kPCR) assay

Journal of Antimicrobial Chemotherapy

Volumn 66, Issue 12, 2011, Pages 2823-2826

  Pirillo, Maria Franca ^a   Recordon Pinson, Patricia ^b   Andreotti, Mauro ^a   Mancini, Maria Grazia ^a   Amici, Roberta ^a   Giuliano, Marina ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

Automated assays; Real time PCR; Resource limited settings

Indexed keywords

VIRUS RNA;

ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; DRIED BLOOD SPOT; GENE DOSAGE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENT; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; QUANTITATIVE ANALYSIS; RNA ANALYSIS; ROOM TEMPERATURE; SENSITIVITY AND SPECIFICITY; STORAGE TEMPERATURE; VIRUS DETECTION; VIRUS LOAD;

AUTOMATION; BLOOD; DESICCATION; HIV INFECTIONS; HIV-1; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIRAL LOAD; VIROLOGY;

EID: 81855199763     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkr383     Document Type: Article

References (11)

1. Brambilla D, Jenning C, Aldrovrandi G et al. Multicenter evaluation of use of dried blood and plasma spot specimens in quantitative assays for human immunodeficiency virus RNA: measurement, precision, and RNA stability. J Clin Microbiol 2003; 41: 1888-93.
2. Waters L, Kambugu A, Tibenderana H et al. Evaluation of filter paper transfer of whole-blood and plasma samples for quantifying HIV RNA in subjects on antiretroviral therapy in Uganda. J Acquir Immune Defic Syndr 2007; 46: 590-3.
3. Johannessen A, Garrido C, Zahonero N et al. Dried blood spots perform well in viral load monitoring of patients who receive antiretroviral treatment in rural Tanzania. Clin Infect Dis 2009; 49: 976-81.
4. Mbida AD, Sosso S, Fleri P et al. Measure of viral load by using the Abbott Real-Time HIV-1 assay on dried blood and plasma spot specimens collected in 2 rural dispensaries in Cameroon. J Acquir Immune Defic Syndr 2009; 52: 9-16.
5. Andreotti M, Pirillo M, Guidotti G et al. Correlation between HIV-1 viral load quantification in plasma, dried blood spots, and dried plasma spots using the Roche COBAS Taqman assay. J Clin Virol 2010; 1: 4-7.
6. van Deursen P, Oosterlaken T, Andre P et al. Measuring human immunodeficiency virus type 1 RNA loads in dried blood spot specimens using NucliSENS EasyQ HIV-1 v2.0. J Clin Virol 2010; 47: 120-5.
7. Battersby T, Kleshik F, Brooks J et al. Analytical performance of an automated HIV-1 dried blood spot assay. In: Abstracts of the European Society for Clinical Virology Winter Meeting, London, 2011. Abstract P12. European Society for Clinical Virology.
8. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986; 327: 307-10.
9. Viljoen J, Gampini S, Danaviah S et al. Dried blood spot HIV-1 RNA quantification using open real-time systems in South Africa and Burkina Faso. J Acquir Immune Defic Syndr 2010; 55: 290-8.
10. Leelawiwat W, Young NL, Chaowanachan T et al. Dried blood spots for the diagnosis and quantitation of HIV-1: stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand. J Virol Methods 2009; 155: 109-17.
11. Monleau M, Butel C, Delaporte E et al. Effect of storage conditions of dried plasma and blood spots in HIV-1 RNA quantification and PCR amplification for drug resistance genotyping. J Antimicrob Chemother 2010; 65: 1582-6.