An improved procedure for the purification of catalytically active alkane hydroxylase from pseudomonas putida GPo1

Applied Biochemistry and Biotechnology

Volumn 165, Issue 3-4, 2011, Pages 823-831

  Xie, Meng ^a,b   Alonso, Hernan ^c   Roujeinikova, Anna ^c  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b MCGILL UNIVERSITY   (Canada)

^c MONASH UNIVERSITY   (Australia)

Author keywords

Alkane biodegradation; Alkane hydroxylase; Biocatalysis; Biomodification of hydrocarbons

Indexed keywords

1-OCTENE; ALKANE HYDROXYLASE; BIOCATALYSIS; BIOMODIFICATION; CATALYSE; EPOXIDATION REACTIONS; HYDROXYLASES; MEMBRANE PROTEINS; MONOOXYGENASES; PSEUDOMONAS PUTIDA; PURITY LEVEL; ROBUST PROCEDURES;

BACTERIA; BIODEGRADATION; BIOLOGICAL MEMBRANES; BIOREMEDIATION; BIOTECHNOLOGY; EPOXIDATION; PURIFICATION; SYNTHESIS (CHEMICAL);

PARAFFINS;

ALKANE HYDROXYLASE; OXYGENASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ENZYME PURIFICATION; EPOXIDATION; NONHUMAN; PSEUDOMONAS PUTIDA;

ALKANE 1-MONOOXYGENASE; ALKANES; ALKENES; BIODEGRADATION, ENVIRONMENTAL; CLONING, MOLECULAR; DIMETHYLAMINES; ESCHERICHIA COLI; HYDROXYLATION; KINETICS; PETROLEUM; PLASMIDS; PROTEIN FOLDING; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS; STEREOISOMERISM; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); PSEUDOMONAS PUTIDA;

EID: 81355138850     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-011-9300-5     Document Type: Article

References (26)

1. LG Whyte A Schultz JB Beilen AP Luz V Pellizari D Labbé, et al. 2002 Prevalence of alkane monooxygenase genes in Arctic and Antarctic hydrocarbon-contaminated and pristine soils FEMS Microbiology Ecology 41 141 150 1:CAS:528:DC%2BD38XmtVWms7Y%3D (Pubitemid 34880052)
2. J van Beilen W Duetz A Schmid B Witholt 2003 Practical issues in the application of oxygenases Trends in Biotechnology 21 170 177 10.1016/S0167-7799(03)00032-5 (Pubitemid 36397985)
3. M Ayala E Torres 2004 Enzymatic activation of alkanes: constraints and prospective Applied Catalysis A 272 1 13 1:CAS:528:DC%2BD2cXmslWjt7Y%3D 10.1016/j.apcata.2004.05.046
4. IE Staijen JB Van Beilen B Witholt 2000 Expression, stability and performance of the three-component alkane mono-oxygenase of Pseudomonas oleovorans in Escherichia coli European Journal of Biochemistry 267 1957 1965 1:CAS:528:DC%2BD3cXisVCisLk%3D 10.1046/j.1432-1327.2000.01196.x (Pubitemid 30185281)
5. J Shanklin E Whittle 2003 Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family FEBS Letters 545 188 192 1:CAS:528:DC%2BD3sXksVOksbY%3D 10.1016/S0014-5793(03)00529-5 (Pubitemid 36694699)
6. JB van Beilen THM Smits FF Roos T Brunner SB Balada M Röthlisberger, et al. 2005 Identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases Journal of Bacteriology 187 85 91 10.1128/JB.187.1.85-91.2005 (Pubitemid 40024183)
7. JB van Beilen EG Funhoff 2007 Alkane hydroxylases involved in microbial alkane degradation Applied Microbiology and Biotechnology 74 13 21 1:CAS:528:DC%2BD2sXhtVansLg%3D 10.1007/s00253-006-0748-0 (Pubitemid 46183463)
8. SW May BJ Abbott 1972 Enzymatic epoxidation. I. Alkene epoxidation by the -hydroxylation system of Pseudomonas oleovorans Biochemical and Biophysical Research Communications 48 1230 1234 1:CAS:528:DyaE38XltlKku7c%3D 10.1016/0006-291X(72)90842-X
9. RD Schwartz CJ McCoy 1973 Pseudomonas oleovorans hydroxylation- epoxidation system: additional strain improvements Applied Microbiology 26 217 218 1:CAS:528:DyaE3sXkslGksb4%3D
10. AG Katopodis HA Smith SW May 1988 New oxyfunctionalization capabilities for omega-hydroxylases-asymmetric aliphatic sulfoxidation and branched ether demethylation Journal of the American Chemical Society 110 897 899 1:CAS:528:DyaL1cXntFKhug%3D%3D 10.1021/ja00211a033
11. B Witholt MJ de Smet J Kingma JB Van Beilen M Kok RG Lageveen, et al. 1990 Bioconversions of aliphatic compounds by Pseudomonas oleovorans in multiphase bioreactors: background and economic potential Trends in Biotechnology 8 46 52 1:CAS:528:DyaK3cXitFGju74%3D 10.1016/0167-7799(90)90133-I
12. EL Johnson MR Hyman 2006 Propane and n-butane oxidation by Pseudomonas putida GPo1 Applied and Environmental Microbiology 72 950 952 1:CAS:528:DC%2BD28XmtFemtQ%3D%3D 10.1128/AEM.72.1.950-952.2006
13. SW May RD Schwartz BJ Abbott OR Zaborsky 1975 Structural effects on reactivity of substrates and inhibitors in epoxidation system of Pseudomonas oleovorans Biochimica et Biophysica Acta 403 245 255 1:CAS:528: DyaE2MXlslCls7g%3D
14. J Shanklin C Achim H Schmidt BG Fox E Münck 1997 Mössbauer studies of alkane omega-hydroxylase: evidence for a diiron cluster in an integral-membrane enzyme Proceedings of the National Academy of Sciences of the United States of America 94 2981 2986 1:CAS:528:DyaK2sXisVKgsbY%3D 10.1073/pnas.94.7.2981 (Pubitemid 27157250)
15. JB van Beilen D Penninga B Witholt 1992 Topology of the membrane-bound alkane hydroxylase of Pseudomonas oleovorans Journal of Biological Chemistry 267 9194 9201
16. EJ McKenna MJ Coon 1970 Enzymatic omega-oxidation. IV. Purification and properties of the omega-hydroxylase of Pseudomonas oleovorans The Journal of Biological Chemistry 245 3882 3889 1:CAS:528:DyaE3cXkslehsro%3D
17. RT Ruettinger ST Olson RF Boyer MJ Coon 1974 Identification of the omega-hydroxylase of Pseudomonas oleovorans as a nonheme iron protein requiring phospholipid for catalytic activity Biochemical and Biophysical Research Communications 57 1011 1017 1:CAS:528:DyaE2cXktlCqtbs%3D 10.1016/0006-291X(74) 90797-9
18. AG Katopodis K Wimalasena J Lee SW May 1984 Mechanistic studies on non-heme iron monooxygenase catalysis-epoxidation, aldehyde formation, and demethylation by the omega-hydroxylation system of Pseudomonas oleovorans Journal of the American Chemical Society 106 7928 7935 1:CAS:528:DyaL2MXisFen 10.1021/ja00337a049
19. M Nieboer J Kingma B Witholt 1993 The alkane oxidation system of Pseudomonas oleovorans: induction of the alk genes in Escherichia coli W3110 (pGEc47) affects membrane biogenesis and results in overexpression of alkane hydroxylase in a distinct cytoplasmic membrane subfraction Molecular Microbiology 8 1039 1051 1:CAS:528:DyaK3sXltlGhu74%3D 10.1111/j.1365-2958.1993. tb01649.x (Pubitemid 23187023)
20. JB van Beilen J Kingma B Witholt 1994 Substrate specificity of the alkane hydroxylase system of Pseudomonas oleovorans Gpo1 Enzyme and Microbial Technology 16 904 911 10.1016/0141-0229(94)90066-3 (Pubitemid 24306288)
21. THM Smits SB Balada B Witholt JB van Beilen 2002 Functional analysis of alkane hydroxylases from gram-negative and gram-positive bacteria Journal of Bacteriology 184 1733 1742 1:CAS:528:DC%2BD38XitFSht70%3D 10.1128/JB.184.6.1733- 1742.2002 (Pubitemid 34188225)
22. G Eggink RG Lageveen B Altenburg B Witholt 1987 Controlled and functional expression of the Pseudomonas oleovorans alkane utilizing system in Pseudomonas putida and Escherichia coli The Journal of Biological Chemistry 262 17712 17718 1:CAS:528:DyaL2sXmtFaqsbg%3D (Pubitemid 18032259)
23. HJ Lee LY Lian NS Scrutton 1997 Recombinant two-iron rubredoxin of Pseudomonas oleovorans: overexpression, purification and characterization by optical, CD and 113Cd NMR spectroscopies Biochemical Journal 328 Pt 1 131 136 1:CAS:528:DyaK2sXotVWnu78%3D
24. MM Bradford 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Analytical Biochemistry 72 248 254 1:CAS:528:DyaE28XksVehtrY%3D 10.1016/0003-2697(76)90527-3
25. MA Andrade P Chacón JJ Merelo F Morán 1993 Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network Protein Engineering 6 383 390 1:CAS:528:DyaK3sXkvFSmsbg%3D 10.1093/protein/6.4.383 (Pubitemid 23197398)
26. JA Cuff GJ Barton 2000 Application of multiple sequence alignment profiles to improve protein secondary structure prediction Proteins 40 502 511 1:CAS:528:DC%2BD3cXlsFCrur8%3D 10.1002/1097-0134(20000815)40:3<502::AID- PROT170>3.0.CO;2-Q (Pubitemid 30624459)