Quantitative Co-expression of proteins at the single cell level - application to a multimeric FRET sensor

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  Goedhart, Joachim ^a   van Weeren, Laura ^a   Adjobo Hermans, Merel J W ^a,b   Elzenaar, Ies ^a   Hink, Mark A ^a   Gadella, Theodorus W J ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CYAN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG; VIRUS 2A PROTEIN; VIRUS PROTEIN; YELLOW FLUORESCENT PROTEIN; BACTERIAL PROTEIN; ENHANCED GREEN FLUORESCENT PROTEIN; G PROTEIN COUPLED RECEPTOR; GREEN FLUORESCENT PROTEIN; PEPTIDE FRAGMENT; PHOTOPROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC HETEROGENEITY; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; INTERNAL RIBOSOME ENTRY SITE; MOLECULAR SENSOR; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN INDUCTION; QUANTITATIVE ANALYSIS; SINGLE CELL ANALYSIS; GENETICS; METABOLISM; REPORTER GENE; RIBOSOME; WESTERN BLOTTING;

BACTERIAL PROTEINS; BLOTTING, WESTERN; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; PEPTIDE FRAGMENTS; PLASMIDS; RECEPTORS, G-PROTEIN-COUPLED; RIBOSOMES; VIRAL PROTEINS;

EID: 81255195819     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027321     Document Type: Article

References (24)

1. Chudakov DM, Lukyanov S, Lukyanov KA, (2005) Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol 23: 605-613.
2. Zhang J, Campbell RE, Ting AY, Tsien RY, (2002) Creating new fluorescent probes for cell biology. Nat Rev Mol Cell Biol 3: 906-918.
3. Miyawaki A, (2003) Visualization of the spatial and temporal dynamics of intracellular signaling. Dev Cell 4: 295-305.
4. Adjobo-Hermans MJ, Goedhart J, van Weeren L, Nijmeijer S, Manders EM, et al. (2011) Real-time visualization of heterotrimeric G protein Gq activation in living cells. BMC Biology 9: 32.
5. Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams JA, et al. (1997) Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388: 882-887.
6. van der Wal J, Habets R, Varnai P, Balla T, Jalink K, (2001) Monitoring agonist-induced phospholipase C activation in live cells by fluorescence resonance energy transfer. J Biol Chem 276: 15337-15344.
7. Miyawaki A, (2011) Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer. Annu Rev Biochem.
8. Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, et al. (2010) Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Nat Methods 7: 137-139.
9. Kremers GJ, Goedhart J, van Munster EB, Gadella TW Jr, (2006) Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Forster radius. Biochemistry 45: 6570-6580.
10. Schwake G, Youssef S, Kuhr JT, Gude S, David MP, et al. (2010) Predictive modeling of non-viral gene transfer. Biotechnol Bioeng 105: 805-813.
11. Martin P, Albagli O, Poggi MC, Boulukos KE, Pognonec P, (2006) Development of a new bicistronic retroviral vector with strong IRES activity. BMC Biotechnol 6: 4.
12. de Felipe P, (2004) Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genet Vaccines Ther 2: 13.
13. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, et al. (2004) Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 22: 589-594.
14. Haustein E, Schwille P, (2007) Fluorescence correlation spectroscopy: novel variations of an established technique. Annu Rev Biophys Biomol Struct 36: 151-169.
15. Wettschureck N, Offermanns S, (2005) Mammalian G proteins and their cell type specific functions. Physiol Rev 85: 1159-1204.
16. Bunemann M, Frank M, Lohse MJ, (2003) Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci U S A 100: 16077-16082.
17. Gibson SK, Gilman AG, (2006) Gialpha and Gbeta subunits both define selectivity of G protein activation by alpha2-adrenergic receptors. Proc Natl Acad Sci U S A 103: 212-217.
18. Janetopoulos C, Jin T, Devreotes P, (2001) Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 291: 2408-2411.
19. Jensen JB, Lyssand JS, Hague C, Hille B, (2009) Fluorescence changes reveal kinetic steps of muscarinic receptor-mediated modulation of phosphoinositides and Kv7.2/7.3 K+ channels. J Gen Physiol 133: 347-359.
20. Hughes TE, Zhang H, Logothetis DE, Berlot CH, (2001) Visualization of a functional Galpha q-green fluorescent protein fusion in living cells. Association with the plasma membrane is disrupted by mutational activation and by elimination of palmitoylation sites, but not be activation mediated by receptors or AlF4. J Biol Chem 276: 4227-4235.
21. Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, et al. (2009) Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 458: 771-775.
22. Gallegos LL, Kunkel MT, Newton AC, (2006) Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. J Biol Chem 281: 30947-30956.
23. Verbeek DS, Goedhart J, Bruinsma L, Sinke RJ, Reits EA, (2008) PKC gamma mutations in spinocerebellar ataxia type 14 affect C1 domain accessibility and kinase activity leading to aberrant MAPK signaling. J Cell Sci 121: 2339-2349.
24. Maeder CI, Hink MA, Kinkhabwala A, Mayr R, Bastiaens PI, et al. (2007) Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol 9: 1319-1326.