Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3′-end maturation and intron degradation

Plant Journal

Volumn 67, Issue 3, 2011, Pages 381-394

  Germain, Arnaud ^a   Herlich, Shira ^b   Larom, Shirley ^b   Kim, Sang Hu ^a   Schuster, Gadi ^b   Stern, David B ^a  

^a United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

chloroplast; intron; polynucleotide phosphorylase; ribonuclease; RNA; splicing

Indexed keywords

ARABIDOPSIS; CATALYTIC SITES; CHLOROPLAST; IN-VITRO; IN-VITRO ANALYSIS; IN-VIVO; INTRON; MUTATIONAL ANALYSIS; PHOSPHOROLYSIS; PLANT ORGANELLES; POLYADENYLATION; POLYNUCLEOTIDE PHOSPHORYLASE; RECOMBINANT ENZYMES; RIBONUCLEASE; TWO-LINE; WILD-TYPE PLANTS;

CATALYST ACTIVITY; CHLOROPHYLL; DEGRADATION; ENZYME ACTIVITY; GENES; PHOSPHORYLATION; PLANTS (BOTANY); POLYMERIZATION; POLYMERS;

RNA;

ARABIDOPSIS PROTEIN; CHLOROPLAST RNA; EXORIBONUCLEASE; MESSENGER RNA; POLYADENYLIC ACID; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; RIBONUCLEASE PH; RIBOSOME RNA;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARTICLE; CHLOROPLAST; CONFORMATION; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; INTRON; METABOLISM; MULTIGENE FAMILY; PHENOTYPE; POINT MUTATION; POLYADENYLATION; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CATALYTIC DOMAIN; CHLOROPLASTS; EXORIBONUCLEASES; INTRONS; MULTIGENE FAMILY; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PHENOTYPE; POINT MUTATION; POLY A; POLYADENYLATION; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; RNA, CHLOROPLAST; RNA, MESSENGER; RNA, RIBOSOMAL;

ARABIDOPSIS;

EID: 80955177475     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/j.1365-313X.2011.04601.x     Document Type: Article

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