Sensitive quantitative assay for point mutations in the rat H-ras gene based on single nucleotide primer extension

Experimental and Therapeutic Medicine

Volumn 1, Issue 4, 2010, Pages 657-661

  Yano, Yoshihisa ^a   Yano, Tomohiro ^b   Kinoshita, Anna ^a   Matoba, Ai ^a   Hasuma, Tadayoshi ^a   Wanibuchi, Hideki ^a   Morimura, Keiichirou ^a   Otani, Shuzo ^a   Fukushima, Shoji ^a  

^a OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b NATIONAL INSTITUTE OF HEALTH AND NUTRITION   (Japan)

Author keywords

2 amino 3,8 dimethylimidazo 4,5 f quinoxaline; H ras; Sensitive quantitative assay for point mutation; Thermosequenase cycle end labeling method

Indexed keywords

ALLELE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOASSAY; CARCINOGENICITY; CONTROLLED STUDY; GENE AMPLIFICATION; IN VIVO STUDY; MALE; NONHUMAN; ONCOGENE H RAS; POINT MUTATION; QUANTITATIVE ASSAY; RAT; SENSITIVITY ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; THERMOSEQUENASE CYCLE END LABELING;

EID: 80855165233     PISSN: 17920981     EISSN: 17921015     Source Type: Journal    
DOI: 10.3892/etm_00000103     Document Type: Article

References (24)

1. Sistonen L and Alitalo K: Activation of c-ras oncogenes by mutations and amplification. Ann Clin Res 18: 297-303, 1986.
2. Orita M, Suzuki Y, Sekiya T, et al: Rapid and sensitive detection of point mutations and DNApolymorphisms using the polymerase chain reaction. Genomics 5: 874-879, 1986.
3. Winter E, Yamamoto F, Almoguera C, et al: Amethod to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells. Proc Natl Acad Sci USA82: 7575-7579, 1985.
4. Myers RM, Lumelsky N, Lerman LS, et al: Detection of single base substitutions in total genomic DNA. Nature 313: 495-498, 1985.
5. Conner BJ, Reyes AA, Morin C, et al: Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides. Proc Natl Acad Sci USA80: 278-282, 1983.
6. Ehlen T and Dubeau L: Detection of ras point mutations by the polymerase chain reaction using mutation-specific, inosine-containing oligonucleotide primers. Biochem Biophys Res Commun 160: 441-447, 1989.
7. Ichikawa T, Yano Y, Uchida M, et al: The activation of K-ras gene at an early stage of lung tumorigenesis in mice. Cancer Lett 107: 165-170, 1996.
8. Yano T, Yuasa M, Murakami A, et al: The detection of chemically initiated cells having the mutation of K-ras gene at an early stage of lung carcinogenesis in mice. Anal Biochem 244: 187-189, 1997.
9. Kawasaki K, Suzuki T, Ueda M, et al: CC to TT mutation in the mitochondrial DNA of normal skin: relationship to ultraviolet light exposure. Mutat Res 22: 35-43, 2000.
10. Takeda S, Ichii S and Nakamura Y: Detection of K-ras mutation in sputum by mutant-allele-specific amplification (MASA). Hum Mutat 2: 112-117, 1993.
11. Liang TJ, Hasegawa K, Munoz SJ, et al: Hepatitis Bvirus precore mutation and fulminant hepatitis in the United States. Apolymerase chain reaction-based assay for the detection of specific mutation. J Clin Invest 93: 550-555, 1994.
12. Horikoshi T, Lenz HJ, Danenberg K, et al: Quantitative determination of the ratio of mutated to normal ras genes in the blood of leukemia patients by allele-specific PCR. Leuk Res 18: 693-702, 1994.
13. Etoh T, Ueo H, Inoue H, et al: Clinical significance of K-ras mutations in intraoperative tumor drainage blood from patients with colorectal carcinoma. Ann Surg Oncol 8: 407-412, 2001.
14. Hashimoto T, Kobayashi Y, Ishikawa Y, et al: Prognostic value of genetically diagnosed lymph node micrometastasis in non-small cell lung carcinoma cases. Cancer Res 60: 6472-6478, 2000.
15. Felton JS and Knize MG: Occurrence, identification, and bacterial mutagenicity of heterocyclic amines in cooked food. Mutat Res 259: 205-217, 1991.
16. Snyderwine EG: Some perspectives on the nutritional aspects of breast cancer research. Food-derived heterocyclic amines as etiologic agents in human mammary cancer. Cancer 74: 1070-1077, 1994.
17. IARC Working Group on the evaluation of carcinogenic risk to humans: MeIQx (2-amino-3,8-demethylimidazo[4,5-f]quinoxaline). In: IARCMonographs on the Evaluation of Carcinogenic Risks to Humans - Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. Vol. 56. Armstrong B(ed). IARCPublisher, Lyon, pp221-228, 1993.
18. Murray S, Gooderham NJ, Boobis AR, et al: Detection and measurement of MeIQx in human urine after ingestion of a cooked meat meal. Carcinogenesis 10: 763-765, 1989.
19. Ushiyama H, Wakabayashi K, Hirose M, et al: Presence of carcinogenic heterocyclic amines in urine of healthy volunteers eating normal diet, but not of inpatients receiving parenteral alimentation. Carcinogenesis 12: 1417-1422, 1991.
20. Tannenbaum SE, Stillwell WG, Ji H, et al: MeIQx (2-amino-3,8-demethylimidazo [4,5-f]quinoxaline): metabolism in humans and urinary metabolites in human populations. In: Heterocyclic Amines in Cooked Foods, Possible Human Carcinogens. Asamson RH, Gustafsson JA, Ito N, Nagao M, Sugimura T, Wakabayashi K and Yamazoe Y (eds). Princeton Scientific Publishing Co. Inc., Princeton, pp197-206, 1993.
21. Totsuka Y, Fukutome K, Takahashi M, et al: Presence of N2-(deoxyguanosin-8-yl)-2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (dG-C8-MeIQx) in human tissues. Carcinogenesis 17: 1029-1034, 1996.
22. Yamashita K, Adachi M, Kato S, et al: DNA adducts formed by 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline in rat liver: dose-response on chronic administration. Jpn JCancer Res 81: 470-476, 1990.
23. Kato T, Ohgaki H, Hasegawa H, et al: Carcinogenicity in rats of a mutagenic compound, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. Carcinogenesis 9: 71-73, 1998
24. Pfau W, Martin FL, Cole KJ, et al: Heterocyclic aromatic amines induce DNA strand breaks and cell transformation. Carcinogenesis 20: 545-551, 1999