Development of a microwell adapted immunoblot system with recombinant antigens for distinguishing human herpesvirus (HHV)6A and HHV6B and detection of human cytomegalovirus

Clinical Chemistry and Laboratory Medicine

Volumn 49, Issue 11, 2011, Pages 1891-1898

  Thäder Voigt, Andrea ^a   Jacobs, Enno ^a   Lehmann, Werner ^b   Bandt, Dirk ^a,c  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b Attomol GmbH   (Germany)

^c Institute of Medical Diagnostics   (Germany)

Author keywords

HCMV; HHV6; Immunoblotting; Microblot; Multiplex detection

Indexed keywords

IMMUNOGLOBULIN G; VIRUS ANTIGEN;

ANTIBODY DETECTION; ARTICLE; BLOTTING; HUMAN; HUMAN CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 6; MICROWELL ADAPTED IMMUNOBLOT SYSTEM; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; SENSITIVITY AND SPECIFICITY; SEROLOGY; VIRUS DETECTION;

ADULT; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; AUTOMATION, LABORATORY; CHILD; CLONING, MOLECULAR; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; HERPESVIRUS 6, HUMAN; HUMANS; IMMUNOBLOTTING; MALE; PLASMIDS; RECOMBINANT PROTEINS; ROSEOLOVIRUS INFECTIONS; SENSITIVITY AND SPECIFICITY; TRANSFORMATION, BACTERIAL; YOUNG ADULT;

HUMAN HERPESVIRUS 5; HUMAN HERPESVIRUS 6; HUMAN HERPESVIRUS 6A;

EID: 80755187931     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2011.666     Document Type: Article

References (43)

1. Söderberg-Nauclér C, Nelson JY. Human cytomegalovirus latency and reactivation - a delicate balance between the virus and its host's immune system. Intervirology 1999;42:314-21. (Pubitemid 30122065)
2. Sarid O, Anson O, Yaari A, Margalith M. Human cytomegalovirus salivary antibodies as related to stress. Clin Lab 2002; 48:297-305. (Pubitemid 34619163)
3. Razonable RR, Fanning C, Brown RA, Espy MJ, Rivero A, Wilson J, et al. Selective reactivation of human herpesvirus 6 variant a occurs in critically ill immunocompetent hosts. J Infect Dis 2002;185:110-3. (Pubitemid 34043024)
4. Vivoli R, Rovesti S, Borella P, Cermelli C. Relation between psychoneuroendocrine profile in stressful conditions and antibodies to herpesvirus 6 and 7. J Biol Regul Homeost Agents 2008;22:239-45.
5. van Son WJ, The TH. Cytomegalovirus infection after organ transplantation: an update with special emphasis on renal transplantation. Transpl Int 1989;2:147-64.
6. Fishman JA, Emery V, Freeman R, Pascual M, Rostaing L, Schlitt HJ, et al. Cytomegalovirus in transplantation - challenging the status quo. Clin Transplant 2007;21:149-58.
7. Ljungman P. Risk assessment in haematopoietic stem cell transplantation: viral status. Best Pract Res Clin Haematol 2007;20: 209-17. (Pubitemid 46585967)
8. Gerna G, Lilleri D. Monitoring transplant patients for human cytomegalovirus: diagnostic update. Herpes 2006;13:4-11. (Pubitemid 43900947)
9. Stocchi R, Ward KN, Fanin R, Baccarani M, Apperley JF. Management of human cytomegalovirus infection and disease after allogeneic bone marrow transplantation. Haematologica 1999; 84:71-9. (Pubitemid 29479384)
10. Hentrich M, Oruzio D, Jager G, Schlemmer M, Schleuning M, Schiel X, et al. Impact of human herpesvirus-6 after haematopoietic stem cell transplantation. Br J Haematol 2005;128:66- 72.
11. Enright H, Haake R, Weisdorf D, Ramsay N, McGlave P, Kersey J, et al. Cytomegalovirus pneumonia after bone marrow transplantation. Risk factors and response to therapy. Transplantation 1993;55:1339-46.
12. Dockrell DH, Paya CV. Human herpesvirus-6 and -7 in transplantation. Rev Med Virol 2001;11:23-36.
13. Yoshikawa T, Asano Y, Ihira M, Suzuki K, Ohashi M, Suga S, et al. Human herpesvirus 6 viremia in bone marrow transplant recipients: clinical features and risk factors. J Infect Dis 2002; 185:847-53. (Pubitemid 34274426)
14. Ablashi DV, Balachandran N, Josephs SF, Hung CL, Krueger GR, Kramarsky B, et al. Genomic polymorphism, growth properties, and immunologic variations in human herpesvirus-6 isolates. Virology 1991;184:545-52.
15. Aubin JT, Agut H, Collandre H, Yamanishi K, Chandran B, Montagnier L, et al. Antigenic and genetic differentiation of the two putative types of human herpes virus 6. J Virol Methods 1993;41:223-34.
16. Hall CB, Caserta MT, Schnabel KC, Long C, Epstein LG, Insel RA, et al. Persistence of human herpesvirus 6 according to site and variant: possible greater neurotropism of variant A. Clin Infect Dis 1998;26:132-7. (Pubitemid 28068166)
17. Dominguez G, Dambaugh TR, Stamey FR, Dewhurst S, Inoue N, Pellett PE. Human herpesvirus 6B genome sequence: coding content and comparison with human herpesvirus 6A. J Virol 1999;73:8040-52. (Pubitemid 29441664)
18. Yamanishi K, Okuno T, Shiraki K, Takahashi M, Kondo T, Asano Y, et al. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet 1988;1:1065-7.
19. Abdel-Haq NM, Asmar BI. Human herpesvirus 6 (HHV6) infection. Indian J Pediatr 2004;71:89-96. (Pubitemid 38209270)
20. Ahlqvist J, Fotheringham J, Akhyani N, Yao K, Fogdell-Hahn A, Jacobson S. Differential tropism of human herpesvirus 6 (HHV-6) variants and induction of latency by HHV-6A in oligodendrocytes. J Neurovirol 2005;11:384-94. (Pubitemid 41614518)
21. Ward KN, Leong HN, Thiruchelvam AD, Atkinson CE, Clark DA. Human herpesvirus 6 DNA levels in cerebrospinal fluid due to primary infection differ from those due to chromosomal viral integration and have implications for diagnosis of encephalitis. J Clin Microbiol 2007;45:1298-304. (Pubitemid 46626501)
22. Dewhurst S, McIntyre K, Schnabel K, Hall CB. Human herpesvirus 6 (HHV-6) variant B accounts for the majority of symptomatic primary HHV-6 infections in a population of U.S. infants. J Clin Microbiol 1993;31:416-8. (Pubitemid 23028652)
23. Caserta MT, McDermott MP, Dewhurst S, Schnabel K, Carnahan JA, Gilbert L, et al. Human herpesvirus 6 (HHV6) DNA persistence and reactivation in healthy children. J Pediatr 2004;145:478-84. (Pubitemid 39345983)
24. Sassenscheidt J, Rohayem J, Illmer T, Bandt D. Detection of b-herpesviruses in allogenic stem cell recipients by quantitative real-time PCR. J Virol Methods 2006;138:40-8. (Pubitemid 44633446)
25. Canzler U, Bartsch H, Ulitzsch S, Kurien B, Dorri Y, Scofield RH, et al. Detection of autoantibodies to tumor-associated antigens in sera of patients with systemic autoimmunity using a novel protein microblot array. Scand J Immunol 2009;69:563-9.
26. Bolt MW, Mahoney PA. High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 1997;247:185-92. (Pubitemid 27200149)
27. Lin W, Kasamatsu H. On the electrotransfer of polypeptides from gels to nitrocellulose membranes. Anal Biochem 1983; 128:302-11.
28. Mattes MJ. Binding parameters of antibodies reacting with multivalent antigens: functional affinity or pseudo-affinity. J Immunol Methods 1997;202:97-101.
29. Zhang H, Williams PS, Zborowski M, Chalmers JJ. Binding affinities/avidities of antibody-antigen interactions: quantification and scale-up implications. Biotechnol Bioeng 2006;95: 812-29. (Pubitemid 44863200)
30. Miller H, McCulloch B, Landini MP, Rossier E. Comparison of immunoblotting with other serological methods and virus isolation for the early detection of primary cytomegalovirus infection in allograft recipients. J Clin Microbiol 1989;27: 2672-7. (Pubitemid 20001326)
31. Okuno T, Takahashi K, Balachandra K, Shiraki K, Yamanishi K, Takahashi M, et al. Seroepidemiology of human herpesvirus 6 infection in normal children and adults. J Clin Microbiol 1989;27:651-3. (Pubitemid 19098097)
32. Wiersbitzky S, Eberle J, Bruns R, Weidemeier H, Bittner S, Dorn U, et al. Seroprevalence of antibodies to human herpesvirus 6 (exanthema subitum; critical 3-day fever-exanthema in young children) in the population of Northern Germany. Kinderarztl Prax 1991;59:170-3.
33. Cone RW, Huang ML, Ashley R, Corey L. Human herpesvirus 6 DNA in peripheral blood cells and saliva from immunocompetent individuals. J Clin Microbiol 1993;31:1262-7. (Pubitemid 23129456)
34. De Bolle L, Naesens L, De Clercq E. Update on human herpesvirus 6 biology, clinical features, and therapy. Clin Microbiol Rev 2005;18:217-45. (Pubitemid 40170670)
35. Cermelli C, Moroni A, Pietrosemoli P, Pecorari M, Portolani M. IgG antibodies to human herpesvirus-6 (HHV-6) in Italian people. Microbiologica 1992;15:57-63.
36. Bhattarakosol P, Pancharoen C, Mekmullica J, Bhattarakosol P. Seroprevalence of anti-human herpesvirus-6 IgG antibody in children of Bangkok, Thailand. Southeast Asian J Trop Med Public Health 2001;32:143-7. (Pubitemid 33643348)
37. Alessio LA, Carricart SE, Bustos D, Nates SV, Gendelman H, Pavan JV. Loss of maternally derived human herpesvirus-6 immunity and natural infection in Argentinian infants. Int J Infect Dis 2001;5:202-4. (Pubitemid 34059581)
38. Enders G, Biber M, Meyer G, Helftenbein E. Prevalence of antibodies to human herpesvirus 6 in different age groups, in children with exanthema subitum, other acute exanthematous childhood diseases, Kawasaki syndrome, and acute infections with other herpesviruses and HIV. Infection 1990;18:12-5. (Pubitemid 20061332)
39. Zerr DM, Meier AS, Selke SS, Frenkel LM, Huang ML, Wald A, et al. A population-based study of primary human herpesvirus 6 infection. N Engl J Med 2005;352:768-76. (Pubitemid 40271171)
40. Boutolleau D, Duros C, Bonnafous P, Caiola D, Karras A, Castro ND, et al. Identification of human herpesvirus 6 variants A and B by primer-specific real-time PCR may help to revisit their respective role in pathology. J Clin Virol 2006;35:257-63. (Pubitemid 43188970)
41. Kim JS, Lee KS, Park JH, Kim MY, Shin WS. Detection of human herpesvirus 6 variant A in peripheral blood mononuclear cells from multiple sclerosis patients. Eur Neurol 2000;43: 170-3.
42. Aberle SW, Mandl CW, Kunz C, Popow-Kraupp T. Presence of human herpesvirus 6 variants A and B in saliva and peripheral blood mononuclear cells of healthy adults. J Clin Microbiol 1996;34:3223-5. (Pubitemid 26389388)
43. Cone RW, Huang ML, Hackman RC, Corey L. Coinfection with human herpesvirus 6 variants A and B in lung tissue. J Clin Microbiol 1996;34:877-81. (Pubitemid 26091499)