2D gel; Database searching; In gel digestion; MALDI TOF; Mass spectrometry; Peptide mass fingerprint; Protein identification; Proteomics; SDS PAGE
Indexed keywords
PEPTIDE FRAGMENT;
PROTEIN;
TRYPSIN;
ALKYLATION;
ARTICLE;
CHEMISTRY;
INSTRUMENTATION;
ISOLATION AND PURIFICATION;
LABORATORY;
LIMIT OF DETECTION;
MASS SPECTROMETRY;
METABOLISM;
METHODOLOGY;
PEPTIDE MAPPING;
PROTEIN DATABASE;
SILVER STAINING;
TWO DIMENSIONAL GEL ELECTROPHORESIS;
Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis
DOI 10.1002/elps.1150060905
Neuhoff V, Stamm R, Eibl H (1985) Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: a systematic analysis. Electrophoresis 6:427-448 (Pubitemid 15023889)
Reduction of alkylation of proteins in preparation of two-dimensional map analysis: Why, when, and how?
DOI 10.1002/1522-2683(200106)22:10<2046::AID-ELPS2046>3.0.CO;2-C
Herbert B, et al (2001) Reduction and alkylation of proteins in preparation of two-dimensional map analysis: Why, when, and how? Electrophoresis 22:2046-2057 (Pubitemid 32621934)
Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels
DOI 10.1021/ac950914h
Shevchenko A, et al (1996) Mass spectrometric sequencing of proteins from silverstained polyacrylamide gels. Anal Chem 68: 850-858 (Pubitemid 26101214)
Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity
Gharahdaghi F, et al (1999) Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis 20:601-605 (Pubitemid 29183427)
Use of matrix clusters and trypsin autolysis fragments as mass calibrants in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
DOI 10.1002/rcm.775
Harris WA, Janecki DJ, Reilly JP (2002) Use of matrix clusters and trypsin autolysis fragments as mass calibrants in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom 16:1714-1722 (Pubitemid 35033728)
Iterative data analysis is the key for exhaustive analysis of peptide mass fingerprints from proteins separated by two-dimensional electrophoresis
DOI 10.1016/S1044-0305(03)00345-3
Schmidt F, et al (2003) Iterative data analysis is the key for exhaustive analysis of peptide mass fingerprints from proteins separated by twodimensional electrophoresis. J Am Soc Mass Spectrom 14:943-956 (Pubitemid 37040756)
