ELISPOT assay for neuroscience research: Studying TNFα secretion from microglial cells

Methods in Molecular Biology

Volumn 792, Issue , 2012, Pages 97-104

  Hagen, Jodi ^a   Houchins, Jeffrey P ^a   Kalyuzhny, Alexander E ^a  

^a RandD Systems Inc   (United States)

Author keywords

BV2 cells; ELISPOT; Microglia; Neuroscience; TNF

Indexed keywords

TUMOR NECROSIS FACTOR ALPHA;

ANIMAL; ARTICLE; CELL CULTURE; ENZYME LINKED IMMUNOSPOT ASSAY; IMMUNOLOGY; METHODOLOGY; MICROGLIA; MOUSE; NEUROSCIENCE; RESEARCH; SECRETION;

ANIMALS; CELLS, CULTURED; ENZYME-LINKED IMMUNOSPOT ASSAY; MICE; MICROGLIA; NEUROSCIENCES; RESEARCH; TUMOR NECROSIS FACTOR-ALPHA;

EID: 80555139550     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-325-7_8     Document Type: Article

References (27)

1. Sedgwick, J. D., and Holt, P. G. (1983) A solidphase immunoenzymatic technique for the enumeration of specific antibody-secreting cells. J Immunol Methods 57, 301-309. (Pubitemid 13116746)
2. Sedgwick, J. D. (2005) ELISPOT assay: a personal retrospective. Methods Mol Biol 302, 3-14.
3. Czerkinsky, C. C., Nilsson, L. A., Nygren, H., Ouchterlony, O., and Tarkowski, A. (1983) A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods 65, 109-121. (Pubitemid 14203438)
4. Kalyuzhny, A. E. (2005) Chemistry and biology of the ELISPOT assay. Methods Mol Biol 302, 15-31.
5. Arlen, P., Tsang, K. Y., Marshall, J. L., Chen, A., Steinberg, S. M., Poole, D., et al.(2000) The use of a rapid ELISPOT assay to analyze peptidespecific immune responses in carcinoma patients to peptide vs. recombinant poxvirus vaccines. Cancer Immunol Immunother 49, 517-529.
6. Asai, T., Storkus, W. J., and Whiteside, T. L. (2000) Evaluation of the modified ELISPOT assay for gamma interferon production in cancer patients receiving antitumor vaccines. Clin Diagn Lab Immunol 7, 145-154. (Pubitemid 30132104)
7. Bienvenu, J., Monneret, G., Fabien, N., and Revillard, J. P. (2000) The clinical usefulness of the measurement of cytokines. Clin Chem Lab Med 38, 267-285. (Pubitemid 30444953)
8. Pass, H. A., Schwarz, S. L., Wunderlich, J. R., and Rosenberg, S. A. (1998) Immunization of patients with melanoma peptide vaccines: immunologic assessment using the ELISPOT assay. Cancer J Sci Am 4, 316-323.
9. Schmittel, A., Keilholz, U., Thiel, E., and Scheibenbogen, C. (2000) Quantification of tumor-specific T lymphocytes with the ELISPOT assay. J Immunother 23, 289-295. (Pubitemid 30327363)
10. Howell, D. M., Feldman, S. B., Kloser, P., and Fitzgerald-Bocarsly, P. (1994) Decreased frequency of functional natural interferon-producing cells in peripheral blood of patients with the acquired immune deficiency syndrome. Clin Immunol Immunopathol 71, 223-230. (Pubitemid 24147241)
11. Chapman, A. L., Munkanta, M., Wilkinson, K. A., Pathan, A. A., Ewer, K., Ayles, H., et al. (2002) Rapid detection of active and latent tuberculosis infection in HIV-positive individuals by enumeration of Mycobacterium tuberculosis-specific T cells. Aids 16, 2285-2293. (Pubitemid 35449014)
12. Kamath, A. T., Groat, N. L., Bean, A. G., and Britton, W. J. (2000) Protective effect of DNA immunization against mycobacterial infection is associated with the early emergence of interferon-gamma (IFN-gamma)-secreting lymphocytes. Clin Exp Immunol 120, 476-482. (Pubitemid 30365553)
13. Dolter, K. E., Evans, C. F., Ellefsen, B., Song, J., Boente-Carrera, M., Vittorino, R., et al. (2010) Immunogenicity, safety, biodistribution and persistence of ADVAX, a prophylactic DNA vaccine for HIV-1, delivered by in vivo electroporation. Vaccine. Epub 2010 Nov 18.
14. Salmon-Ceron, D., Durier, C., Desaint, C., Cuzin, L., Surenaud, M., Hamouda, N. B., et al. (2010) Immunogenicity and safety of an HIV-1 lipopeptide vaccine in healthy adults: a phase 2 placebo-controlled ANRS trial. AIDS 24, 2211-2223.
15. Tansey, M. G., and Goldberg, M. S. (2009) Neuroinflammation in Parkinson's disease: its role in neuronal death and implications for therapeutic intervention. Neurobiol Dis 37, 510-518. Epub 2009 Nov 10.
16. Park, K. M., and Bowers, W. J. (2010) Tumor necrosis factor-alpha mediated signaling in neuronal homeostasis and dysfunction. Cell 22, 977-983. Epub 2010 Jan 21.
17. Cunningham, A. J., Murray, C. A., O'Neill, L. A., Lynch, M. A., and O'Connor, J. J. (1996) Interleukin-1 beta (IL-1 beta) and tumour necrosis factor (TNF) inhibit long-term potentiation in the rat dentate gyrus in vitro. Neurosci Lett 203, 17-20.
18. Pickering, M., Cumiskey, D., and O'Connor, J. J. (2005) Actions of TNF-alpha on glutamatergic synaptic transmission in the central nervous system. Exp Physiol 90, 663-670. Epub 2005 Jun 8. (Pubitemid 41406053)
19. Pickering, M., and O'Connor, J. J. (2007) Proinflammatory cytokines and their effects in the dentate gyrus. Prog Brain Res 163, 339-354. (Pubitemid 47321444)
20. Tran, T. A., McCoy, M. K., Sporn, M. B., and Tansey, M. G. (2008) The synthetic triterpenoid CDDO-methyl ester modulates microglial activities, inhibits TNF production, and provides dopaminergic neuroprotection. J Neuroinflammation 5, 14.
21. Chakraborty, S., Kaushik, D. K., Gupta, M., and Basu, A. (2010) Inflammasome signaling at the heart of central nervous system pathology. J Neurosci Res 88, 1615-1631.
22. Vallejo, R., Tilley, D. M., Vogel, L., and Benyamin, R. (2010) The role of glia and the immune system in the development and maintenance of neuropathic pain. Pain 10, 167-184. Epub 2010 Apr 5.
23. Spicarova, D., and Palecek, J. (2010) Tumor necrosis factor alpha sensitizes spinal cord TRPV1 receptors to the endogenous agonist N-oleoyldopamine J Neuroinflammation 7, 49.
24. Austin, P. J., and Moalem-Taylor, G. (2010) The neuro-immune balance in neuropathic pain: Involvement of inflammatory immune cells, immune-like glial cells and cytokines. J Neuroimmunology 229, 26-50. Epub 2010 Sep 25.
25. Blasi, E., Barluzzi, R., Bocchini, V., Mazzolla, R., and Bistoni, F. (1990) Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus. J Neuroimmunol 27, 229-237. (Pubitemid 20146292)
26. Hald, A., and Lotharius, J. (2005) Oxidative stress and inflammation in Parkinson's disease: is there a causal link? Exp Neurol 193, 279-90. (Pubitemid 40616179)
27. Kalyuzhny, A., and Stark, S. (2001) A simple method to reduce the background and improve well-to-well reproducibility of staining in ELISPOT assays J Immunol Methods 257, 93-7. (Pubitemid 33009356)