Bacterial ATP-driven transporters of transition metals: Physiological roles, mechanisms of action, and roles in bacterial virulence

Metallomics

Volumn 3, Issue 11, 2011, Pages 1098-1108

  Klein, Joshua S ^a   Lewinson, Oded ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ABC TRANSPORTER; ANTIBACTERIAL DRUGS; ATP HYDROLYSIS; ATPASES; BACTERIAL PATHOGENS; CELLULAR FUNCTION; DRIVEN SYSTEM; INTRACELLULAR CONCENTRATION; INTRACELLULAR LEVELS; METAL HOMEOSTASIS; P-TYPE; SPECIFIC TASKS; STRUCTURAL DIFFERENCES; TOXIC METALS; TRANSPORT PROTEINS;

BACTERIOLOGY; ION EXCHANGE; MANGANESE; METALS; PHYSIOLOGY; STRUCTURAL METALS; TOXIC MATERIALS;

COPPER;

ABC TRANSPORTER; BACTERIAL PROTEIN; CADMIUM; COBALT; COPPER; COPPER EXPORTING ADENOSINE TRIPHOSPHATASE; CYANOCOBALAMIN; LEAD; MERCURY; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE; SILVER; TRANSITION ELEMENT; ZINC;

BACTERIAL VIRULENCE; ENZYME SPECIFICITY; HUMAN; HYDROLYSIS; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PROTEIN TRANSPORT; REVIEW;

ADENOSINE TRIPHOSPHATE; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BACTERIA; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; MODELS, MOLECULAR; PROTEIN CONFORMATION; VITAMIN B 12;

BACTERIA (MICROORGANISMS); PROKARYOTA;

EID: 80455163144     PISSN: 17565901     EISSN: 1756591X     Source Type: Journal    
DOI: 10.1039/c1mt00073j     Document Type: Review

References (98)

1. C. Andreini I. Bertini G. Cavallaro G. Holliday J. Thornton Metal ions in biological catalysis: from enzyme databases to general principles JBIC, J. Biol. Inorg. Chem. 2008 13 8 1205 1218
2. K. J. Waldron J. C. Rutherford D. Ford N. J. Robinson Metalloproteins and metal sensing Nature 2009 460 7257 823 830
3. K. Helbig C. Grosse D. H. Nies Cadmium toxicity in glutathione mutants of Escherichia coli J. Bacteriol. 2008 190 15 5439 5454
4. K. H. Thompson C. Orvig Boon and bane of metal ions in medicine Science 2003 300 5621 936 939 (Pubitemid 36548871)
5. D. H. Nies Efflux-mediated heavy metal resistance in prokaryotes FEMS Microbiol. Rev. 2003 27 2-3 313 339 (Pubitemid 36700434)
6. E.-H. Kim D. H. Nies M. M. McEvoy C. Rensing Switch or Funnel: How RND-Type Transport Systems Control Periplasmic Metal Homeostasis J. Bacteriol. 2011 193 10 2381 2387
7. E. L. Borths K. P. Locher A. T. Lee D. C. Rees The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter Proc. Natl. Acad. Sci. U. S. A. 2002 99 26 16642 16647 (Pubitemid 36034026)
8. V. Braun F. Endriss Energy-coupled outer membrane transport proteins and regulatory proteins BioMetals 2007 20 3 219 231 (Pubitemid 46776586)
9. D. M. Carter I. R. Miousse J.-N. Gagnon â. Martinez A. Clements J. Lee M. A. Hancock H. Gagnon P. D. Pawelek J. W. Coulton Interactions between TonB from Escherichia coli and the Periplasmic Protein FhuD J. Biol. Chem. 2006 281 46 35413 35424 (Pubitemid 46036579)
10. K. J. James M. A. Hancock J.-N. Gagnon J. W. Coulton TonB Interacts with BtuF, the Escherichia coli Periplasmic Binding Protein for Cyanocobalamin Biochemistry 2009 48 39 9212 9220
11. D. W. Christianson J. D. Cox Catalysis by metal-activated hydroxide in zinc and manganese metalloenzymes Annu. Rev. Biochem. 1999 68 1 33 57 (Pubitemid 29449186)
12. D. L. Huffman T. V. O'Halloran Function, structure, and mechanism of copper trafficking proteins Annu. Rev. Biochem. 2001 70 1 677 701 (Pubitemid 32662222)
13. K. D. Karlin Metalloenzymes, structural motifs, and inorganic models Science 1993 261 5122 701 708 (Pubitemid 23278384)
14. A. F. Parisi B. L. Vallee Zinc Metalloenzymes: Characteristics and Significance in Biology and Medicine Am. J. Clin. Nutr. 1969 22 9 1222 1239
15. K. Orino L. Lehman Y. Tsuji H. Ayaki S. V. Torti F. M. Torti Ferritin and the response to oxidative stress Biochem. J. 2001 357 1 241 247 (Pubitemid 32642922)
16. C. Blindauer Bacterial metallothioneins: past, present, and questions for the future J. Biol. Inorg. Chem 2011 1 14
17. R. Sharma C. Rensing B. P. Rosen B. Mitra The ATP hydrolytic activity of purified ZntA, a Pb(ii)/Cd(ii)/Zn(ii)-translocating ATPase from Escherichia coli J. Biol. Chem. 2000 275 6 3873 3878 (Pubitemid 30094611)
18. C. Rensing B. Fan R. Sharma B. Mitra B. P. Rosen CopA: An Escherichia coli Cu(i)-translocating P-type ATPase Proc. Natl. Acad. Sci. U. S. A. 2000 97 2 652 656 (Pubitemid 30070364)
19. K. Hollenstein D. C. Frei K. P. Locher Structure of an ABC transporter in complex with its binding protein Nature 2007 446 7132 213 216 (Pubitemid 46398672)
20. M. T. Sebulsky B. H. Shilton C. D. Speziali D. E. Heinrichs The Role of FhuD2 in Iron(iii)-Hydroxamate Transport in Staphylococcus aureus J. Biol. Chem. 2003 278 50 49890 49900
21. J. M. Arguello Identification of ion-selectivity determinants in heavy-metal transport P1B-type ATPases J. Membr. Biol. 2003 195 2 93 108 (Pubitemid 37243987)
22. M. S. Francis C. J. Thomas Mutants in the CtpA copper transporting P-type ATPase reduce virulence of Listeria monocytogenes Microb. Pathog. 1997 22 2 67 78 (Pubitemid 27151802)
23. O. Lewinson A. T. Lee D. C. Rees A P-type ATPase importer that discriminates between essential and toxic transition metals Proc. Natl. Acad. Sci. U. S. A. 2009 106 12 4677 4682
24. S. Tottey S. A. Rondet G. P. Borrelly P. J. Robinson P. R. Rich N. J. Robinson A copper metallochaperone for photosynthesis and respiration reveals metal-specific targets, interaction with an importer, and alternative sites for copper acquisition J. Biol. Chem. 2002 277 7 5490 7 (Pubitemid 34968599)
25. S. Tottey P. R. Rich S. A. Rondet N. J. Robinson Two Menkes-type atpases supply copper for photosynthesis in Synechocystis PCC 6803 J. Biol. Chem. 2001 276 23 19999 20004
26. D. Kahn M. David O. Domergue M. L. Daveran J. Ghai P. R. Hirsch J. Batut Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation J. Bacteriol. 1989 171 2 929 939
27. B. K. Hassani C. Astier W. Nitschke S. Ouchane CtpA, a Copper-translocating P-type ATPase Involved in the Biogenesis of Multiple Copper-requiring Enzymes J. Biol. Chem. 2010 285 25 19330 19337
28. H.-G. Koch C. Winterstein A. S. Saribas J. O. Alben F. Daldal Roles of the ccoGHIS gene products in the biogenesis of the cbb3-type cytochrome c oxidase J. Mol. Biol. 2000 297 1 49 65
29. O. Preisig R. Zufferey H. Hennecke The Bradyrhizobium japonicum fixGHIS genes are required for the formation of the high-affinity cbb3-type cytochrome oxidase Arch. Microbiol. 1996 165 5 297 305 (Pubitemid 26175058)
30. + efflux ATPases in Pseudomonas aeruginosa Mol. Microbiol. 2010 78 5 1246 1258
31. S. J. Dutta J. Liu A. J. Stemmler B. Mitra Conservative and Nonconservative Mutations of the Transmembrane CPC Motif in ZntA: Effect on Metal Selectivity and Activity Biochemistry 2007 46 12 3692 3703 (Pubitemid 46493470)
32. B. Fan B. P. Rosen Biochemical characterization of CopA, the Escherichia coli Cu(i)-translocating P-type ATPase J. Biol. Chem. 2002 277 49 46987 46992 (Pubitemid 36159204)
33. 2+-ATPase from Listeria monocytogenes J. Biol. Chem. 2006 281 40 29533 29541 (Pubitemid 44536961)
34. 2+-ATPase of Listeria monocytogenes Biochem. J. 2003 369 3 681 685 (Pubitemid 36246253)
35. B. Mitra R. Sharma The cysteine-rich amino-terminal domain of ZntA, a Pb(ii)/Zn(ii)/Cd(ii)-translocating ATPase from Escherichia coli, is not essential for its function Biochemistry 2001 40 25 7694 7699 (Pubitemid 32578033)
36. Z.-j. Hou S. Narindrasorasak B. Bhushan B. Sarkar B. Mitra Functional Analysis of Chimeric Proteins of the Wilson Cu(i)-ATPase (ATP7B) and ZntA, a Pb(ii)/Zn(ii)/Cd(ii)-ATPase from Escherichia coli J. Biol. Chem. 2001 276 44 40858 40863 (Pubitemid 37373232)
37. B. Fan G. Grass C. Rensing B. P. Rosen Escherichia coli CopA N-Terminal Cys(X)2Cys Motifs Are Not Required for Copper Resistance or Transport Biochem. Biophys. Res. Commun. 2001 286 2 414 418 (Pubitemid 32924930)
38. C.-C. Wu W. J. Rice D. L. Stokes Structure of a Copper Pump Suggests a Regulatory Role for Its Metal-Binding Domain Structure (London) 2008 16 6 976 985 (Pubitemid 351778383)
39. R. A. Pufahl C. P. Singer K. L. Peariso S. J. Lin P. J. Schmidt C. J. Fahrni V. C. Culotta J. E. Penner-Hahn T. V. O'Halloran Metal Ion Chaperone Function of the Soluble Cu(i) Receptor Atx1 Science 1997 278 5339 853 856 (Pubitemid 27467921)
40. J. M. Walker R. Tsivkovskii S. Lutsenko Metallochaperone Atox1 Transfers Copper to the NH2-terminal Domain of the Wilson's Disease Protein and Regulates Its Catalytic Activity J. Biol. Chem. 2002 277 31 27953 27959 (Pubitemid 34966743)
41. + to transmembrane transport sites Proc. Natl. Acad. Sci. U. S. A. 2008 105 16 5992 5997 (Pubitemid 351758387)
42. N. S. Kadaba J. T. Kaiser E. Johnson A. Lee D. C. Rees The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation Science 2008 321 250 253 (Pubitemid 351989096)
43. S. Gerber M. Comellas-Bigler B. A. Goetz K. P. Locher Structural basis of trans-inhibition in a molybdate/tungstate ABC transporter Science 2008 321 246 250 (Pubitemid 351989095)
44. M. K. Doeven R. Abele R. Tampe B. Poolman The binding specificity of OppA determines the selectivity of the oligopeptide ATP-binding cassette transporter J. Biol. Chem. 2004 279 31 32301 32307 (Pubitemid 39014681)
45. N. Cadieux C. Bradbeer E. Reeger-Schneider W. Koster A. K. Mohanty M. C. Wiener R. J. Kadner Identification of the Periplasmic Cobalamin-binding Protein BtuF of Escherichia coli J. Bacteriol. 2002 184 3 706 717 (Pubitemid 34150217)
46. M. R. Rohrbach V. Braun W. Koster Ferrichrome transport in Escherichia coli K-12: altered substrate specificity of mutated periplasmic FhuD and interaction of FhuD with the integral membrane protein FhuB J. Bacteriol. 1995 177 24 7186 7193
47. O. Lewinson A. T. Lee K. P. Locher D. C. Rees A distinct mechanism for the ABC transporter BtuCD-BtuF revealed by the dynamics of complex formation Nat. Struct. Mol. Biol. 2010 17 3 332 338
48. E. L. Borths B. Poolman R. N. Hvorup K. P. Locher D. C. Rees In vitro Functional Characterization of BtuCD-F, the Escherichia coli ABC Transporter for Vitamin B12 Uptake Biochemistry 2005 44 49 16301 16309 (Pubitemid 41785829)
49. P. Gourdon X.-Y. Liu T. Skjorringe J. P. Morth L. B. Moller B. P. Pedersen P. Nissen Crystal structure of a copper-transporting PIB-type ATPase Nature 2011 475 7354 59 64
50. +-transporting PIB-type ATPases J. Biol. Chem. 2004 279 52 54802 54807 (Pubitemid 40053225)
51. S. J. Dutta J. Liu Z. Hou B. Mitra Conserved aspartic acid 714 in transmembrane segment 8 of the ZntA subgroup of P1B-type ATPases is a metal-binding residue Biochemistry 2006 45 18 5923 5931
52. +-transporting ATPase J. Biol. Chem. 2007 282 35 25213 25221 (Pubitemid 47372814)
53. W. W. Ho H. Li S. Eakanunkul Y. Tong A. Wilks M. Guo T. L. Poulos Holo- and apo-bound structures of bacterial periplasmic heme-binding proteins J. Biol. Chem. 2007 282 49 35796 35802 (Pubitemid 350277105)
54. J. B. Neilands Siderophores: Structure and Function of Microbial Iron Transport Compounds J. Biol. Chem. 1995 270 45 26723 26726
55. C. Linke T. T. Caradoc-Davies P. G. Young T. Proft E. N. Baker The Laminin-Binding Protein Lbp from Streptococcus pyogenes is a Zinc Receptor J. Bacteriol. 2009 191 18 5814 5823
56. 2+ in Cyanobacteria is Redox Controlled J. Mol. Biol. 2005 348 4 961 969 (Pubitemid 40552796)
57. R. Albers Biochemical aspects of active transport Annu. Rev. Biochem. 1967 36 727 756
58. R. L. Post C. Hegyvary S. Kume Activation by Adenosine Triphosphate in the Phosphorylation Kinetics of Sodium and Potassium Ion Transport Adenosine Triphosphatase J. Biol. Chem. 1972 247 20 6530 6540
59. J. Argüello E. Eren M. González-Guerrero The structure and function of heavy metal transport P1B-ATPases BioMetals 2007 20 3 233 248 (Pubitemid 46776570)
60. M. Bublitz H. Poulsen J. P. Morth P. Nissen In and out of the cation pumps: P-Type ATPase structure revisited Curr. Opin. Struct. Biol. 2010 20 4 431 439
61. P. L. Jorgensen K. O. Håkansson S. J. D. Karlish Structure and Mechanism of Na, K-ATPASE: Functional Sites and Their Interactions Annu. Rev. Physiol. 2003 65 1 817 849 (Pubitemid 38249178)
62. 2+-ATPase pumps ions across the sarcoplasmic reticulum membrane Biochim. Biophys. Acta, Mol. Cell Res. 2009 1793 6 941 946
63. G. F.-L. Ames C. E. Liu A. K. Joshi K. Nikaido Liganded and Unliganded Receptors Interact with Equal Affinity with the Membrane Complex of Periplasmic Permeases, a Subfamily of Traffic ATPases J. Biol. Chem. 1996 271 24 14264 14270 (Pubitemid 26191304)
64. J. S. Patzlaff T. van der Heide B. Poolman The ATP/substrate stoichiometry of the ATP-binding cassette (ABC) transporter OpuA J. Biol. Chem. 2003 278 32 29546 29551 (Pubitemid 36962336)
65. K. Nikaido G. F.-L. Ames One Intact ATP-binding Subunit is Sufficient to Support ATP Hydrolysis and Translocation in an ABC Transporter, the Histidine Permease J. Biol. Chem. 1999 274 38 26727 26735
66. I. L. Urbatsch L. Beaudet I. Carrier P. Gros Mutations in Either Nucleotide-Binding Site of P-glycoprotein (Mdr3) prevent Vanadate Trapping of Nucleotide at Both SitesĆ Biochemistry 1998 37 13 4592 4602 (Pubitemid 28217177)
67. R. Ernst P. Kueppers C. M. Klein T. Schwarzmueller K. Kuchler L. Schmitt A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5 Proc. Natl. Acad. Sci. U. S. A. 2008 105 13 5069 5074 (Pubitemid 351738511)
68. +-ATPase from the Extremophile Archaeoglobus fulgidus J. Biol. Chem. 2002 277 9 7201 7208 (Pubitemid 34953111)
69. A. Bateman E. Birney R. Durbin S. R. Eddy K. L. Howe E. L. Sonnhammer The Pfam protein families database Nucleic Acids Res. 2000 28 1 263 266 (Pubitemid 30047775)
70. H. M. Berman J. Westbrook Z. Feng G. Gilliland T. N. Bhat H. Weissig I. N. Shindyalov P. E. Bourne The Protein Data Bank Nucleic Acids Res. 2000 28 1 235 242 (Pubitemid 30047768)
71. S. Henikoff E. A. Greene S. Pietrokovski P. Bork T. K. Attwood L. Hood Gene families: the taxonomy of protein paralogs and chimeras Science 1997 278 5338 609 614 (Pubitemid 27464952)
72. R. P. Berntsson S. H. Smits L. Schmitt D. J. Slotboom B. Poolman A structural classification of substrate-binding proteins FEBS Lett. 2010 584 12 2606 2617
73. E. Dassa P. Bouige The ABC of ABCS: a phylogenetic and functional classification of ABC systems in living organisms Res. Microbiol. 2001 152 3-4 211 229 (Pubitemid 32436481)
74. A. L. Davidson E. Dassa C. Orelle J. Chen Structure, function, and evolution of bacterial ATP-binding cassette systems Microbiol. Mol. Biol. Rev. 2008 72 2 317 364 (Pubitemid 351822295)
75. D. C. Rees E. Johnson O. Lewinson ABC transporters: the power to change Nat. Rev. Mol. Cell Biol. 2009 10 3 218 227
76. K. P. Locher B. Rees R. Koebnik A. Mitschler L. Moulinier J. P. Rosenbusch D. Moras Transmembrane Signaling across the Ligand-Gated FhuA Receptor: Crystal Structures of Free and Ferrichrome-Bound States Reveal Allosteric Changes Cell 1998 95 6 771 778 (Pubitemid 29014457)
77. C. Bradbeer P. R. Reynolds G. M. Bauler M. T. Fernandez A requirement for calcium in the transport of cobalamin across the outer membrane of Escherichia coli J. Biol. Chem. 1986 261 6 2520 2523 (Pubitemid 17218257)
78. R. Annamalai B. Jin Z. Cao S. M. Newton P. E. Klebba Recognition of ferric catecholates by FepA J. Bacteriol. 2004 186 11 3578 3589 (Pubitemid 38661556)
79. K. Postle R. A. Larsen TonB-dependent energy transduction between outer and cytoplasmic membranes BioMetals 2007 20 3-4 453 465 (Pubitemid 46776585)
80. J. Chen S. Sharma F. A. Quiocho A. L. Davidson Trapping the transition state of an ATP-binding cassette transporter: evidence for a concerted mechanism of maltose transport Proc. Natl. Acad. Sci. U. S. A. 2001 98 4 1525 1530 (Pubitemid 32165532)
81. V. Braun C. Herrmann Docking of the periplasmic FecB binding protein to the FecCD transmembrane proteins in the ferric citrate transport system of Escherichia coli J. Bacteriol. 2007 189 19 6913 6918 (Pubitemid 47525435)
82. W. R. Schwan P. Warrener E. Keunz C. K. Stover K. R. Folger Mutations in the cueA gene encoding a copper homeostasis P-type ATPase reduce the pathogenicity of Pseudomonas aeruginosa in mice Int. J. Med. Microbiol. 2005 295 4 237 242 (Pubitemid 41020797)
83. S. K. Ward B. Abomoelak E. A. Hoye H. Steinberg A. M. Talaat CtpV: a putative copper exporter required for full virulence of Mycobacterium tuberculosis Mol. Microbiol. 2010 77 5 1096 1110
84. 2+ Uptake System ZnuABC is Required for Bacterial Zinc Homeostasis in Intracellular Environments and Contributes to the Virulence of Salmonella enterica Infect. Immun. 2007 75 12 5867 5876 (Pubitemid 350207826)
85. S. W. Bearden R. D. Perry The Yfe system of Yersinia pestis transports iron and manganese and is required for full virulence of plague Mol. Microbiol. 1999 32 2 403 414 (Pubitemid 29178705)
86. D. Brem C. Pelludat A. Rakin C. A. Jacobi J. Heesemann Functional analysis of yersiniabactin transport genes of Yersinia enterocolitica Microbiology (Reading, U. K.) 2001 147 Pt 5 1115 1127 (Pubitemid 32454630)
87. J. S. Brown S. M. Gilliland D. W. Holden A Streptococcus pneumoniae pathogenicity island encoding an ABC transporter involved in iron uptake and virulence Mol. Microbiol. 2001 40 3 572 585 (Pubitemid 32453111)
88. J. D. Fetherston V. J. Bertolino R. D. Perry YbtP and YbtQ: two ABC transporters required for iron uptake in Yersinia pestis Mol. Microbiol. 1999 32 2 289 299 (Pubitemid 29178696)
89. O. Gat I. Mendelson T. Chitlaru N. Ariel Z. Altboum H. Levy S. Weiss H. Grosfeld S. Cohen A. Shafferman The solute-binding component of a putative Mn(ii) ABC transporter (MntA) is a novel Bacillus anthracis virulence determinant Mol. Microbiol. 2005 58 2 533 551 (Pubitemid 41476167)
90. R. Janulczyk S. Ricci L. Bjorck MtsABC is Important for Manganese and Iron Transport Oxidative Stress Resistance, and Virulence of Streptococcus pyogenes, Infection and Immunity 2003 71 5 2656 2664
91. A. Marra S. Lawson J. S. Asundi D. Brigham A. E. Hromockyj In vivo characterization of the psa genes from Streptococcus pneumoniae in multiple models of infection Microbiology 2002 148 Pt 5 1483 1491 (Pubitemid 34567226)
92. S. Paik A. Brown C. L. Munro C. N. Cornelissen T. Kitten The sloABCR operon of Streptococcus mutans encodes an Mn and Fe transport system required for endocarditis virulence and its Mn-dependent repressor J. Bacteriol. 2003 185 20 5967 5975 (Pubitemid 37248448)
93. T. Pattery J. P. Hernalsteens H. De Greve Identification and molecular characterization of a novel Salmonella enteritidis pathogenicity islet encoding an ABC transporter Mol. Microbiol. 1999 33 4 791 805 (Pubitemid 29392659)
94. G. M. Rodriguez I. Smith Identification of an ABC transporter required for iron acquisition and virulence in Mycobacterium tuberculosis J. Bacteriol. 2006 188 2 424 430 (Pubitemid 43100529)
95. M. L. Zaharik V. L. Cullen A. M. Fung S. J. Libby S. L. Kujat Choy B. Coburn D. G. Kehres M. E. Maguire F. C. Fang B. B. Finlay The Salmonella enterica serovar typhimurium divalent cation transport systems MntH and SitABCD are essential for virulence in an Nramp1G169 murine typhoid model Infect. Immun. 2004 72 9 5522 5525 (Pubitemid 39223447)
96. D. S. Ong L. Wang Y. Zhu B. Ho J. L. Ding The response of ferritin to LPS and acute phase of Pseudomonas infection J. Endotoxin Res. 2005 11 5 267 280 (Pubitemid 41568112)
97. M. F. Cellier P. Courville C. Campion Nramp1 phagocyte intracellular metal withdrawal defense Microbes Infect. 2007 9 14-15 1662 1670 (Pubitemid 350225742)
98. H. S. Garmory R. W. Titball ATP-binding cassette transporters are targets for the development of antibacterial vaccines and therapies Infect. Immun. 2004 72 12 6757 6763 (Pubitemid 39552342)