Actin co-sedimentation assay; for the analysis of protein binding to F-Actin

Journal of Visualized Experiments

Volumn , Issue 13, 2008, Pages

  Srivastava, Jyoti ^a   Barber, Diane ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Biochemistry; F actin; In vitro binding; Issue 13; Protein; Ultracentrifugation

Indexed keywords

EID: 80355148332     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/690     Document Type: Article

References (3)

1. Senetar, M.A., Foster S.J., McCann R.O. Intrasteric inhibition mediates the interaction of the I/LWEQ module proteins Talin1, Talin2, Hip1, and Hip12 with actin. Biochemistry. 14;43(49):15418-28 (2004).
2. Goldmann, W.H., Hess, D., Isenberg, G. The effect of intact talin and talin tail fragment on actin filament dynamics and structure depends on pH and ionic strength. Eur J Biochem. 260(2):439-45 (1999).
3. Lee, H., Bellin, R.M., Walker, D.L., Patel, B., Powers, P., Liu, H., Garcia-Alvarez, B., de Pereda, J., Liddington, R.C., Volkmann, N., Hanein, D., Critchley, D.R. and Robson, R.M. Characterization of an Actin-binding Site within the Talin FERM Domain. J Mol Biol. 22;343(3):771-84 (2004).