Automated system for single molecule fluorescence measurements of surface-immobilized biomolecules

Journal of Visualized Experiments

Volumn , Issue 33, 2009, Pages

  Fiori, Nicolas Di ^a   Meller, Amit ^a,b  

^a BOSTON UNIVERSITY   (United States)

^b Boston University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IMMOBILIZED PROTEIN; NUCLEIC ACID;

ARTICLE; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; METHODOLOGY; MICROFLUIDIC ANALYSIS;

FLUORESCENCE RESONANCE ENERGY TRANSFER; IMMOBILIZED PROTEINS; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NUCLEIC ACIDS;

EID: 80355147837     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1542     Document Type: Article

References (7)

1. Selvin, P. R & Ha, T. Single-Molecule Techniques, a Laboratory Manual. CSHL Press (2008).
2. Sabanayagam, C. R, Eid, J.S. & Meller, A. High-throughput scanning confocal microscope for single molecule analysis. Applied Pys. Letters. 84-7, 1216-1218, (2004).
3. Sabanayagam, C. R, Eid, J.S. & Meller, A. Using fluorescence resonance energy transfer to measure distances along individual DNA molecules: Corrections due to nonideal transfer. J. Chem. Phys. 122, 61103-61105, (2004).
4. Echeverra Aitken, C., Marshall, R.A., Puglisi, J.D. An Oxygen Scavenging System for Improvement of Dye Stability in Single-Molecule Fluorescence Experiments. Biophysical Journal 94, 1826-1835 (2008).
5. Rasnik, I., McKinney, S.A., Ha, T. Nonblinking and long-lasting single molecule fluorescence imaging. Nature Methods 3-11 891-893 (2006).
6. Boukobza, E., Sonnenfeld, A., Haran, G. Immobilization in Surface-Tethered Lipid Vesicles as a New Tool for Single Biomolecule Spectroscopy. J. Phys. Chem. B 105, 12165-12170 (2001).
7. Di Fiori, N., Meller, A. (in preparation)