Fabrication of micro-tissues using modules of collagen gel containing cells

Journal of Visualized Experiments

Volumn , Issue 46, 2010, Pages

  Dean Chamberlain, M ^a   Butler, Mark J ^a   Ciucurel, Ema C ^a   Fitzpatrick, Lindsay E ^a   Khan, Omar F ^a   Leung, Brendan M ^a   Lo, Chuen ^a   Patel, Ritesh ^a   Velchinskaya, Alexandra ^a   Voice, Derek N ^a   Sefton, Michael V ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

3D tissue culture; Bioengineering; Collagen gels; Endothelial cells; Issue 46; Micro tissue; Modules; Tissue engineering

Indexed keywords

EID: 80355146149     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2177     Document Type: Article

References (11)

1. Chamberlain, M. D., Gupta, R. & Sefton, M. V. Chimeric vessel tissue engineering driven by endothelialized modules. (2010).
2. Corstorphine, L. E. & Sefton, M. V. Effectiveness factor and diffusion limitations in collagen gel modules containing HepG2 cells. J Tissue Eng Regen Med (2010).
3. Gupta, R., Van Rooijen, N. & Sefton, M. V. Fate of endothelialized modular constructs implanted in an omental pouch in nude rats. Tissue Eng Part A 15, 2875-87 (2009).
4. Leung, B. M. & Sefton, M. V. A modular tissue engineering construct containing smooth muscle cells and endothelial cells. Ann Biomed Eng 35, 2039-49 (2007).
5. McGuigan, A. P., Leung, B. & Sefton, M. V. Fabrication of cell-containing gel modules to assemble modular tissue-engineered constructs [corrected]. Nat Protoc 1, 2963-9 (2006).
6. McGuigan, A. P. & Sefton, M. V. Vascularized organoid engineered by modular assembly enables blood perfusion. Proc Natl Acad Sci U S A 103, 11461-6 (2006).
7. McGuigan, A. P. & Sefton, M. V. Modular tissue engineering: fabrication of a gelatin-based construct. J Tissue Eng Regen Med 1, 136-45 (2007).
8. McGuigan, A. P. & Sefton, M. V. Design and fabrication of sub-mm-sized modules containing encapsulated cells for modular tissue engineering. Tissue Eng 13, 1069-78 (2007).
9. McGuigan, A. P. & Sefton, M. V. Design criteria for a modular tissue-engineered construct. Tissue Eng 13, 1079-89 (2007).
10. McGuigan, A. P. & Sefton, M. V. The thrombogenicity of human umbilical vein endothelial cell seeded collagen modules. Biomaterials 29, 2453-63 (2008).
11. Sosnik, A., Leung, B., McGuigan, A. P. & Sefton, M. V. Collagen/poloxamine hydrogels: cytocompatibility of embedded HepG2 cells and surface-attached endothelial cells. Tissue Eng 11, 1807-16 (2005).