Increasing cDNA yields from single-cell quantities of mRNA in standard laboratory reverse transcriptase reactions using acoustic microstreaming

Journal of Visualized Experiments

Volumn , Issue 53, 2011, Pages

  Boon, Wah Chin ^a   Petkovic Duran, Karolina ^b   Zhu, Yonggang ^b   Manasseh, Richard ^c   Horne, Malcolm K ^a   Aumann, Tim D ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b CSIRO   (Australia)

^c SWINBURNE UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

Acoustic microstreaming; Bioengineering; Brain; Cells; Gene expression; Issue 53; Microfluidics; Micromixer; Neuroscience; qPCR; Reverse transcription

Indexed keywords

EID: 80355145759     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3144     Document Type: Article

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