Mouse in utero electroporation: Controlled spatiotemporal gene transfection

Journal of Visualized Experiments

Volumn , Issue 54, 2011, Pages

  Matsui, Asuka ^a   Yoshida, Aya C ^a   Kubota, Mayumi ^a   Ogawa, Masaharu ^a   Shimogori, Tomomi ^a  

^a RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355145620     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3024     Document Type: Article

References (22)

1. Tabata, H. & Nakajima, K. Efficient in utero gene transfer system to the developing mouse brain using electroporation: Visualization of neuronal migration in the developing cortex. Neuroscience. 103, 865-872 (2001).
2. Saito, T. & Nakatsuji, N. Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Developmental Biology. 240, 237-246, doi:10.1006/dbio.2001.0439 (2001).
3. Fukuchi-Shimogori, T. & Grove, E. A. Neocortex patterning by the secreted signaling molecule FGF8. Science. 294, 1071-1074 (2001).
4. Kataoka, A. & Shimogori, T. Fgf8 controls regional identity in the developing thalamus. Development. 135, 2873-2881, doi:10.1242/dev.021618 (2008).
5. Imayoshi, I., Shimogori, T., Ohtsuka, T. & Kageyama, R. Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline. Development. 135, 2531-2541, doi:10.1242/dev.021535 (2008).
6. Shimogori, T., Banuchi, V., Ng, H. Y., Strauss, J. B. & Grove, E. A. Embryonic signaling centers expressing BMP, WNT and FGF proteins interact to pattern the cerebral cortex. Development. 131, 5639-5647, doi:10.1242/dev.01428 (2004).
7. Shimogori, T. & Ogawa, M. Gene application with in utero electroporation in mouse embryonic brain. Development Growth & Differentiation. 50, 499-506 doi:10.1111/j.1440-169x.2008.01045.x (2008).
8. Takahashi, Y., Watanabe, T., Nakagawa, S., Kawakami, K. & Sato, Y. in Avian Embryology, 2nd Edition Vol. 87 Methods in Cell Biology 271 (Elsevier Academic Press Inc, 2008).
9. Feil, R. et al. Ligand-activated site-specific recombination in mice. Proceedings of the National Academy of Sciences of the United States of America. 93, 10887-10890 (1996).
10. Indra, A. K. et al. Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase activity of the tamoxifen-inducible Cre-ERT and Cre-ERT2 recombinases. Nucleic Acids Research. 27, 4324-4327 (1999).
11. Nakagawa, Y. & O'Leary, D. D. M. Dynamic patterned expression of orphan nuclear receptor genes ROR alpha and ROR beta in developing mouse forebrain. Developmental Neuroscience. 25, 234-244, doi:10.1159/000072271 (2003).
12. Tabata, H. & Nakajima, K. Efficient in utero gene transfer system to the developing mouse brain using electroporation: Visualization of neuronal migration in the developing cortex. Neuroscience. 103, 865-872 (2001).
13. Saito, T. & Nakatsuji, N. Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Developmental Biology. 240, 237-246, doi:10.1006/dbio.2001.0439 (2001).
14. Fukuchi-Shimogori, T. & Grove, E. A. Neocortex patterning by the secreted signaling molecule FGF8. Science. 294, 1071-1074 (2001).
15. Kataoka, A. & Shimogori, T. Fgf8 controls regional identity in the developing thalamus. Development. 135, 2873-2881, doi:10.1242/dev.021618 (2008).
16. Imayoshi, I., Shimogori, T., Ohtsuka, T. & Kageyama, R. Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline. Development. 135, 2531-2541, doi:10.1242/dev.021535 (2008).
17. Shimogori, T., Banuchi, V., Ng, H. Y., Strauss, J. B. & Grove, E. A. Embryonic signaling centers expressing BMP, WNT and FGF proteins interact to pattern the cerebral cortex. Development. 131, 5639-5647, doi:10.1242/dev.01428 (2004).
18. Shimogori, T. & Ogawa, M. Gene application with in utero electroporation in mouse embryonic brain. Development Growth & Differentiation. 50, 499-506, doi:10.1111/j.1440-169x.2008.01045.x (2008).
19. Takahashi, Y., Watanabe, T., Nakagawa, S., Kawakami, K. & Sato, Y. in Avian Embryology, 2nd Edition Vol. 87 Methods in Cell Biology 271 (Elsevier Academic Press Inc, 2008).
20. Feil, R. et al. Ligand-activated site-specific recombination in mice. Proceedings of the National Academy of Sciences of the United States of America. 93, 10887-10890 (1996).
21. Indra, A. K. et al. Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase activity of the tamoxifen-inducible Cre-ERT and Cre-ERT2 recombinases. Nucleic Acids Research. 27, 4324-4327 (1999).
22. Nakagawa, Y. & O'Leary, D. D. M. Dynamic patterned expression of orphan nuclear receptor genes ROR alpha and ROR beta in developing mouse forebrain. Developmental Neuroscience. 25, 234-244, doi:10.1159/000072271 (2003).