Analysis of pluripotent stem cells by using cryosections of embryoid bodies

Journal of Visualized Experiments

Volumn , Issue 46, 2010, Pages

  Gomes, Ismael C ^a   Acquarone, Mariana ^a   de Moraes Maciel, Renata ^a   Erlich, Rafael Bierig ^a   Rehen, Stevens K ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Cryosections; Developmental Biology; Embryoid body; Embryonic stem cells; H9; Immunochytochemistry; Issue 46

Indexed keywords

EID: 80355145313     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2344     Document Type: Article

References (10)

1. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., & Jones, J.M. Embryonic stem cell lines derived from human blastocysts. Science. 282, 1145-7 (1998).
2. Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Eden, A., Yanuka, O., Amit, M., Soreq, H., & Benvenisty, N. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med. 6. 88-95 (2000).
3. Kurosawa, H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. J Biosci Bioeng. 103, 389-398 (2007).
4. Conley, B.J., Young, J.C., Trounson, A.O., & Mollard, R. Derivation, propagation and differentiation of human embryonic stem cells. Int J Biochem Cell Biol. 36. 555-567 (2004).
5. Moon, I.S., Cho, S.J., Jin, I., Walikonis, R. A simple method for combined fluorescence in situ hybridization and immunocytochemistry. Mol Cells. 24(1):76-82 (2007).
6. Daneshtalab, N., Doré JJ, Smeda JS. Troubleshooting tissue specificity and antibody selection: Procedures in immunohistochemical studies. J Pharmacol Toxicol Methods. 61(2):127-35 (2009).
7. Krenacs, L., Krenacs T, Stelkovics E, Raffeld M. Heat-induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections. Methods Mol Biol. 588:103-19 (2010).
8. Nones, J., Spohr TC, Furtado DR, Sartore RC, Paulsen BS, Guimarães MZ, Rehen SK. Cannabinoids modulate cell survival in embryoid bodies. Cell Biol Int. 12. 399-408 (2010).
9. Huang, W.M., Gibson SJ, Facer P, Gu J, Polak JM. Improved section adhesion for immunocytochemistry using high molecular weight polymers of L-lysine as a slide coating. Histochemistry. 77(2):275-9 (1983).
10. Kuenzi, M.J. and Sherwood OD. Immunohistochemical localization of specific relaxin-binding cells in thecervix, mammary glands, and nipples of pregnant rats. Endocrinology. 136: 1367-1373 (1995).