NanoDrop microvolume quantitation of nucleic acids

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Desjardins, Philippe ^a   Conklin, Deborah ^a  

^a Thermo Scientific NanoDrop Products   (United States)

Author keywords

Basic Protocols; DNA A260; DNA Quantification; DNA Quantitation; Fluorescence PicoGreen; Issue 45; Microvolume Fluorometer; Microvolume Quantitation; Microvolume Spectrophotometer; NanoDrop; Nucleic Acid Quantitation; RNA Quantification

Indexed keywords

EID: 80355142439     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2565     Document Type: Article

References (4)

1. Wilfinger, W.W., Mackey, K., & Chomczynski, P. Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity. BioTechniques. 22:474-481 (1997).
2. Voolstra, C., Jungnickel, A., Borrmann, L., Kirchner, R., & Huber, A. Spectrophotometric Quantification of Nucleic Acids: LabelGuard enables photometric quantification of submicroliter samples using a standard photometer. Implen Applications Note, Munich, Germany. (2006).
3. Ingle Jr, J.D., & Crouch, S.R. Spectrochemical analysis. XV + 590 pp. Prentice Hall, Englewood Cliffs, NJ. (1988).
4. Van Lancker, M. and Gheyssens, L.C. A comparison of four frequently used assays for quantitative determination of DNA. Anal. Lett. 19:615-623 (1986).