Cell electrofusion visualized with fluorescence microscopy

Journal of Visualized Experiments

Volumn , Issue 41, 2010, Pages

  Trontelj, Katja ^a   Ušaj, Marko ^a   Miklavčič, Damijan ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355142171     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1991     Document Type: Article

References (10)

1. Čemazar, M., Jarm, T., Miklavčič, D., Maček-Lebar, A., Ihan, A., Kopitar, N.A., Serša, G. Effect of electric-field intensity on electropermeabilization and electrosensitivity of various tumor-cell lines in vitro. Electro. Magnetobiol 17, 263-272 (1998).
2. Trontelj, K., Reberšek, M., Kandušer, M., Čurin Šerbec, V., Šprohar, M., Miklavčič, D. Optimization of bulk cell electrofusion in vitro for production of human-mouse heterohybridoma cells. Bioelectrochemistry 74, 124-129 (2008).
3. Rols, M.-P. and Teissié, J. Modulation of electrically induced permeabilization and fusion of Chinese hamster ovary cells by osmotic pressure. Biochemistry 29, 4561-4567 (1990).
4. Neil, G. and Zimmermann, U. Electrofusion. in Methods in Enzymology, vol.220, p.174-196
5. Abidor, I.G., Li, L.-H., Hui, S.W. Studies of cell pellets: II. Osmotic properties, electroporation, and related phenomena: Membrane interactions. Biophysical journal 67, 427-435 (1994).
6. Jaroszeski, M.J., Gilbert, R., Fallon, P.G., Heller, R. Mechanically facilitated cell-cell electrofusion. Biophys J. 67(4), 1574-1581 (1994).
7. Ramos, C., Bonefant, D., Teissié J. Cell hybridization by electrofusion on filters. Analytical biochemistry 302, 213-219 (2002).
8. Cao, Y., Yang, J., Yin, Z.Q., et al. Study of high-throughput cell electrofusion in a microelectrode-array chip. Microfluid Nanofluid 5, 669-675 (2008).
9. Ušaj, M., Trontelj, K., Kandušer, M., Miklavčič, D. Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization. Radiol. Oncol. 43, 108-119 (2009).
10. Teissie, J. and Ramos, C. Correlation between electric field pulse induced long-lived permeabilization and fusogenicity in cell membranes. Biophys. J. 74, 1889-1898 (1998).