Isolation and in vitro activation of caenorhabditis elegans sperm

Journal of Visualized Experiments

Volumn , Issue 47, 2010, Pages

  Singaravelu, Gunasekaran ^a   Chatterjee, Indrani ^a   Marcello, Matthew R ^a   Singson, Andrew ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

Nematode; Protease; Pseudopod; Spermatid; Spermatozoa; Spermiogenesis

Indexed keywords

EID: 80355142016     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2336     Document Type: Article

References (12)

1. Kimble, J. & Crittenden, S. L. Controls of germline stem cells, entry into meiosis, and the Sperm/Oocyte decision in Caenorhabditis elegans. Annual Review of Cell and Developmental Biology 23, 405-433, (2007).
2. Lhernault, S. W. & Roberts, T. M. in Methods in Cell Biology, Vol 48 Vol. 48 Methods in Cell Biology 273-301 (1995).
3. Hodgkin, J., Horvitz, H. & Brenner, S. Nondisjunction Mutants of the Nematode Caenorhabditis elegans. Genetics 91, 67-94 (1979).
4. Altun, Z.F. and Hall, D.H. Introduction to male C. elegans anatomy. In WormAtlas, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, (2008).
5. Shakes, D. & Ward, S. Initiation of spermiogenesis in C. elegans: a pharmacological and genetic analysis. Dev Biol 134, 189-200, (1989).
6. Nelson, G. A. & Ward, S. Vesicle fusion, pseudopod extension and amoeboid motility are induced in nematode spermatids by the ionophore monensin. Cell 19, 457-464 (1980).
7. L'Hernault, S. W. The genetics and cell biology of spermatogenesis in the nematode C. elegans. Molecular and Cellular Endocrinology 306, 59-65, (2009).
8. Chatterjee, I., Richmond, A., Putiri, E., Shakes, D. & Singson, A. The Caenorhabditis elegans spe-38 gene encodes a novel four-pass integral membrane protein required for sperm function at fertilization. Development 132, 2795-2808, (2005).
9. Xu, X. & Sternberg, P. A C. elegans sperm TRP protein required for sperm-egg interactions during fertilization. Cell 114, 285-297, (2003).
10. Nelson, G., Roberts, T. & Ward, S. Caenorhabditis elegans spermatozoan locomotion: amoeboid movement with almost no actin. J Cell Biol 92, 121-131 (1982).
11. Machaca, K., DeFelice, L. J., & L'Hernault, S. W. A novel chloride channel localizes to Caenorhabditis elegans spermatids and chloride channel blockers induce spermatid differentiation. Dev Biol 176, 1-16, (1996).
12. LaMunyon, C. & Ward, S. Assessing the viability of mutant and manipulated sperm by artificial insemination of Caenorhabditis elegans. Genetics 138, 689-692 (1994).