In vivo imaging of intact Drosophila larvae at sub-cellular resolution

Journal of Visualized Experiments

Volumn , Issue 43, 2010, Pages

  Zhang, Yao ^a,b   Füger, Petra ^a   Hannan, Shabab B ^a,b   Kern, Jeannine V ^a   Lasky, Bronwen ^a   Rasse, Tobias M ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Anesthetization; Basic Protocols; Desflurane; Development; Drosophila; Imaging; In vivo; Issue 43; Microscopy; Neuromuscular; Synapse

Indexed keywords

EID: 80355142001     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2249     Document Type: Article

References (8)

1. Rasse, T.M. et al. Glutamate receptor dynamics organizing synapse formation in vivo. Nat Neurosci. 8, 898-905 (2005).
2. Rasse, T. M. In vivo imaging of long-term changes in the Drosophila neuromuscular system, Dissertation, Georg-August-University Göttingen, (2004).
3. Fuger, P., Behrends, L. B., Mertel, S., Sigrist, S. J. & Rasse, T. M. Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses. Nat Protoc 2, 3285-3298 (2007).
4. Wang, X. & Schwarz, T. L. Imaging axonal transport of mitochondria. Methods in enzymology 457, 319-333 (2009).
5. Vinegoni, C. et al. Mesoscopic fluorescence tomography for in-vivo imaging of developing Drosophila. J Vis Exp (2009).
6. Lin, C. Y. et al. Label-free imaging of Drosophila larva by multiphoton autofluorescence and second harmonic generation microscopy. Journal of biomedical optics 13, 050502 (2008).
7. Levitan, E. S., Lanni, F. & Shakiryanova, D. In vivo imaging of vesicle motion and release at the Drosophila neuromuscular junction. Nat Protoc 2, 1117-1125 (2007).
8. Zito, K., Parnas, D., Fetter, R. D., Isacoff, E. Y. & Goodman, C. S. Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila. Neuron 22, 719-729 (1999).