Transurethral induction of mouse urinary tract infection

Journal of Visualized Experiments

Volumn , Issue 42, 2010, Pages

  Thai, Kim H ^a   Thathireddy, Anuradha ^b   Hsieh, Michael H ^b  

^a STANFORD UNIVERSITY   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bacteria; Bacterial; Cystitis; Issue 42; Mice; Microbiology; Mouse; Pyelonephritis; Urethra; Urethral; Urinary tract infection; UTI

Indexed keywords

EID: 80355140485     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2070     Document Type: Article

References (13)

1. Schaeffer, A. J., Schwan, W. R., Hultgren, S. J. & Duncan, J. L. Relationship of type 1 pilus expression in Escherichia coli to ascending urinary tract infections in mice. Infect Immun 55, 373-380 (1987).
2. Rouschop, K. M. et al. Urothelial CD44 facilitates Escherichia coli infection of the murine urinary tract. J Immunol 177, 7225-7232 (2006).
3. Malaviya, R., Ikeda, T. & Abraham, S. N. Contribution of mast cells to bacterial clearance and their proliferation during experimental cystitis induced by type 1 fimbriated E. coli. Immunol Lett M 91, 103-111, (2004).
4. Lane, M. C., Alteri, C. J., Smith, S. N. & Mobley, H. L. Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract. Proc Natl Acad Sci U S A 104, 16669-16674 (2007).
5. Hvidberg, H. et al. Development of a long-term ascending urinary tract infection mouse model for antibiotic treatment studies. Antimicrob Agents Chemother 44, 156-163 (2000).
6. Hopkins, W. J., Hall, J. A., Conway, B. P. & Uehling, D. T. Induction of urinary tract infection by intraurethral inoculation with Escherichia coli: refining the murine model. J Infect Dis 171, 462-465 (1995).
7. Hopkins, W. J., Gendron-Fitzpatrick, A., Balish, E. & Uehling, D. T. Time course and host responses to Escherichia coli urinary tract infection in genetically distinct mouse strains. Infect Immun 66, 2798-2802 (1998).
8. Gunther, N. W. t., Lockatell, V., Johnson, D. E. & Mobley, H. L. In vivo dynamics of type 1 fimbria regulation in uropathogenic Escherichia coli during experimental urinary tract infection. Infect Immun 69, 2838-2846 (2001).
9. Bishop, B. L. et al. Cyclic AMP-regulated exocytosis of Escherichia coli from infected bladder epithelial cells. Nat Med 13, 625-630 (2007).
10. Thumbikat, P., Waltenbaugh, C., Schaeffer, A. J. & Klumpp, D. J. Antigen-specific responses accelerate bacterial clearance in the bladder. J Immunol 176, 3080-3086 (2006).
11. Hagberg, L. et al. Ascending, unobstructed urinary tract infection in mice caused by pyelonephritogenic Escherichia coli of human origin. Infect Immun 40, 273-283 (1983).
12. Hopkins, W. J., Hall, J. A., Conway, B. P. & Uehling, D. T. Induction of urinary tract infection by intraurethral inoculation with Escherichia coli: refining the murine model. J Infect Dis 171, 462-465 (1995).
13. Hung, C. S., Dodson, K. W. & Hultgren, S. J. A murine model of urinary tract infection. Nat Protoc 4, 1230-1243 (2009).