Igy technology: Extraction of chicken antibodies from egg yolk by polyethylene glycol (PEG) precipitation

Journal of Visualized Experiments

Volumn , Issue 51, 2011, Pages

  Pauly, Diana ^a   Chacana, Pablo A ^b   Calzado, Esteban G ^c   Brembs, Björn ^d   Schade, Rüdiger ^e  

^a ROBERT KOCH INSTITUTE   (Germany)

^b CICVyA INTA Castelar   (Germany)

^c CENTER OF MOLECULAR IMMUNOLOGY   (Cuba)

^d FREIE UNIVERSITÄT BERLIN   (Germany)

^e CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Flow Cytometry; Immunology; Intratracheal; Issue 51; Lung Draining Lymph Nodes; Lungs; Mouse

Indexed keywords

EID: 80355139317     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3084     Document Type: Article

References (8)

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4. Pauly, D., Dorner, M., Zhang, X., Hlinak, A., Dorner, B. & Schade, R. Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period. Poultry Science 88, 281-290 (2009).
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7. Schade, R., Calzado, E.G., Sarmiento, R., Chacana, P.A., Porankiewicz-Asplund, J. & Terzolo, H.R. Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine. Altern. Lab Anim. 33, 129-154 (2005).
8. Witkowski, P.T., Bourquain, D.R., Hohn, O., Schade, R. & Nitsche, A. Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specifiv IgY antibodies. J. Immunol. Methods 341, 146-153 (2009)