Dissection and staining of drosophila larval ovaries

Journal of Visualized Experiments

Volumn , Issue 51, 2011, Pages

  Maimon, Iris ^a   Gilboa, Lilach ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Cellular biology; Development; Dissection; Drosophila; Germ line stem cells; Issue 51; Larvae; Niche; Ovaries

Indexed keywords

EID: 80355139297     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2537     Document Type: Article

References (19)

1. Fuller, M.T. & Spradling, A.C. Male and female Drosophila germline stem cells: two versions of immortality.Science (New York, N.Y 316, 402-404 (2007).
2. Li, L. & Xie, T. Stem cell niche: structure and function. Annual review of cell and developmental biology 21, 605-631 (2005).
3. Chen, D. & McKearin, D. Gene circuitry controlling a stem cell niche. Curr Biol 15, 179-184 (2005).
4. Kai, T. & Spradling, A. An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells. Proceedings of the National Academy of Sciences of the United States of America 100, 4633-4638 (2003).
5. Kai, T. & Spradling, A. Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries. Nature 428, 564-569 (2004).
6. Lopez-Onieva, L., Fernandez-Minan, A. & Gonzalez-Reyes, A. Jak/Stat signalling in niche support cells regulates dpp transcription to control germline stem cell maintenance in the Drosophila ovary. Development (Cambridge, England) 135, 533-540 (2008).
7. Song, X., Zhu, C.H., Doan, C. & Xie, T. Germline stem cells anchored by adherens junctions in the Drosophila ovary niches. Science (New York, N.Y 296, 1855-1857 (2002).
8. Tazuke, S.I. et al. A germline-specific gap junction protein required for survival of differentiating early germ cells. Development (Cambridge, England) 129, 2529-2539 (2002).
9. Xie, T. & Spradling, A.C. decapentaplegic is essential for the maintenance and division of germline stem cells in the Drosophila ovary. Cell 94, 251-260 (1998).
10. Xie, T. & Spradling, A.C. A niche maintaining germ line stem cells in the Drosophila ovary. Science (New York, N.Y 290, 328-330 (2000).
11. Guo, Z. & Wang, Z. The glypican Dally is required in the niche for the maintenance of germline stem cells and short-range BMP signaling in the Drosophila ovary. Development (Cambridge, England) 136, 3627-3635 (2009).
12. Wang, X., Harris, R.E., Bayston, L.J. & Ashe, H.L. Type IV collagens regulate BMP signalling in Drosophila. Nature 455, 72-77 (2008).
13. Godt, D. & Laski, F.A. Mechanisms of cell rearrangement and cell recruitment in Drosophila ovary morphogenesis and the requirement of bric a brac. Development (Cambridge, England) 121, 173-187 (1995).
14. Sahut-Barnola, I., Godt, D., Laski, F.A. & Couderc, J.L. Drosophila ovary morphogenesis: analysis of terminal filament formation and identification of a gene required for this process. Developmental biology 170, 127-135 (1995).
15. Song, X., Call, G.B., Kirilly, D. & Xie, T. Notch signaling controls germline stem cell niche formation in the Drosophila ovary. Development (Cambridge, England) 134, 1071-1080 (2007).
16. Zhu, C.H. & Xie, T. Clonal expansion of ovarian germline stem cells during niche formation in Drosophila.Development (Cambridge, England) 130, 2579-2588 (2003).
17. Ward, E.J. et al. Stem cells signal to the niche through the Notch pathway in the Drosophila ovary. Curr Biol 16, 2352-2358 (2006).
18. Gilboa, L. & Lehmann, R. Repression of primordial germ cell differentiation parallels germ line stem cell maintenance. Curr Biol 14, 981-986 (2004).
19. Gilboa, L. & Lehmann, R. Soma-germline interactions coordinate homeostasis and growth in the Drosophila gonad. Nature 443, 97-100 (2006).