Measuring diffusion coefficients via two-photon fluorescence recovery after photobleaching

Journal of Visualized Experiments

Volumn , Issue 36, 2010, Pages

  Sullivan, Kelley D ^a   Brown, Edward B ^b  

^a UNIVERSITY OF ROCHESTER   (United States)

^b University of Rochester   (United States)

Author keywords

Cellular biology; Diffusion; Fluorescence recovery after photobleaching; FPR; Issue 36; MP FRAP; Multi photon

Indexed keywords

EID: 80355138839     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1636     Document Type: Article

References (3)

1. Denk, W., Strickler, J.H., and Webb, W.W. Two-photon laser scanning fluorescence microscopy. Biophys J. 60, 73-76 (1990).
2. Brown, E.B., Wu, E.S., Zipfel, W., and Webb W.W. Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery. Biophys J. 77, 2837-2849 (1999).
3. Sullivan, K.D., Sipprell, W.H. III, Brown, E.B. Jr., Brown, E.B. III. Improved model of fluorescence recovery expands the application of multiphoton fluorescence recover after photobleaching in vivo. Biophys J. 96, 5082-5094 (2009).