Purification of the M. magneticum strain AMB-1 magnetosome associated protein MamAΔ41

Journal of Visualized Experiments

Volumn , Issue 37, 2010, Pages

  Zeytuni, Natalie ^a   Zarivach, Raz ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Cellular biology; Issue 37; Magnetosome; Magnetotactic bacteria; MamA; Recombinant protein purification

Indexed keywords

EID: 80355135531     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1844     Document Type: Article

References (15)

1. Faivre, D. & Schuler, D. Magnetotactic Bacteria and Magnetosomes, Chem Rev 108, 4875-4898 (2008).
2. D'Andrea, L. D. & Regan, L. TPR proteins: the versatile helix, Trends Biochem Sci 28, 655-662 (2003).
3. Young, J. C., Barral, J. M. & Ulrich Hartl, F. More than folding: localized functions of cytosolic chaperones, Trends Biochem Sci 28, 541-547(2003).
4. Brocard, C. & Hartig, A. Peroxisome targeting signal 1: is it really a simple tripeptide?, Biochim Biophys Acta 1763, 1565-1573 (2006).
5. Fransen, M., Amery, L., Hartig, A., Brees, C., Rabijns, A., Mannaerts, G. P. & Van Veldhoven, P. P. Comparison of the PTS1- and Rab8b-binding properties of Pex5p and Pex5Rp/TRIP8b, Biochim Biophys Acta 1783, 864-873 (2008).
6. Baker, M. J., Frazier, A. E., Gulbis, J. M. & Ryan, M. T. Mitochondrial protein-import machinery: correlating structure with function, Trends Cell Biol 17, 456-464 (2007).
7. Mirus, O., Bionda, T., von Haeseler, A. & Schleiff, E. Evolutionarily evolved discriminators in the 3-TPR domain of the Toc64 family involved in protein translocation at the outer membrane of chloroplasts and mitochondria, J Mol Model 15, 971-982 (2009).
8. Gatsos, X., Perry, A. J., Anwari, K., Dolezal, P., Wolynec, P. P., Likic, V. A., Purcell, A. W., Buchanan, S. K. & Lithgow, T. Protein secretion and outer membrane assembly in Alphaproteobacteria, FEMS Microbiol Rev 32, 995-1009 (2008).
9. Tiwari, D., Singh, R. K., Goswami, K., Verma, S. K., Prakash, B., and Nandicoori, V. K. Key residues in Mycobacterium tuberculosis protein kinase G play a role in regulating kinase activity and survival in the host, J Biol Chem 284, 27467-27479 (2009).
10. Edqvist, P. J., Broms, J. E., Betts, H. J., Forsberg, A., Pallen, M. J. & Francis, M. S. Tetratricopeptide repeats in the type III secretion chaperone, LcrH: their role in substrate binding and secretion, Mol Microbiol 59, 31-44 (2006).
11. Grunberg, K., Muller, E. C., Otto, A., Reszka, R., Linder, D., Kube, M., Reinhardt, R. & Schuler, D. Biochemical and proteomic analysis of the magnetosome membrane in Magnetospirillum gryphiswaldense, Appl Environ Microbiol 70, 1040-1050 (2004).
12. Komeili, A., Vali, H., Beveridge, T. J. & Newman, D. K. Magnetosome vesicles are present before magnetite formation, and MamA is required for their activation, Proc Natl Acad Sci USA 101, 3839-3844 (2004).
13. Okuda, Y. & Fukumori, Y. Expression and characterization of a magnetosome-associated protein, TPR-containing MAM22, in Escherichia coli, FEBS Lett 491, 169-173 (2001).
14. Taoka, A., Asada, R., Sasaki, H., Anzawa, K., Wu, L. F. & Fukumori, Y. Spatial localizations of Mam22 and Mam12 in the magnetosomes of Magnetospirillum magnetotacticum, J Bacteriol 188, 3805-3812 (2006).
15. Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expression and Purification 41,207 234 (2005).