Detection of Neu1 sialidase activity in regulating TOLL-like receptor activation

Journal of Visualized Experiments

Volumn , Issue 43, 2010, Pages

  Amith, Schammim R ^a   Jayanth, Preethi ^a   Finlay, Trisha ^a   Franchuk, Susan ^a   Gilmour, Alanna ^a   Abdulkhalek, Samar ^a   Szewczuk, Myron R ^a  

^a QUEEN S UNIVERSITY   (Canada)

Author keywords

Cell signaling; Cellular Biology; Fluorescence microscopy; Issue 43; Macrophages; Neu1 sialidase; Receptor activation; Sialidase substrate; TOLL like receptors

Indexed keywords

EID: 80355133835     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2142     Document Type: Article

References (6)

1. Amith, S. R. et al. Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling. Cell Signal 22 314-324, (2010).
2. Amith, S. R. et al. Dependence of pathogen molecule-induced Toll-like receptor activation and cell function on Neu1 sialidase. Glycoconjugate Journal 26, 1197-1212, (2009).
3. Woronowicz, A. et al. Dependence of neurotrophic factor activation of Trk tyrosine kinase receptors on cellular sialidase. Glycobiology 17, 10-24, (2007).
4. Jayanth, P., Amith, S.R., Gee, K. and Szewczuk, M.R. Neu1 Sialidase and Matrix Metalloproteinase-9 Cross-talk is Essential for Neurotrophin Activation of Trk Receptors and Cellular Signaling. Cell. Signal. 22, 1193-1205 (2010).
5. Finlay, T. M., Jayanth, P., Amith, S.R., Glimour, A., Guzzo, C., Gee, K., Beyaert, R., Szewczuk, M.R. Thymoquinone from nutraceutical black cumin oil activates Neu4 sialidase in live macrophage, dendritic, and normal and type I sialidosis human fibroblast cells via GPCR Gαi proteins and matrix metalloproteinase-9. Glycoconj J 27, 329-348 (2010).
6. Seyrantepe, V. et al. Regulation of Phagocytosis in Macrophages by Neuraminidase 1. Journal of Biological Chemistry 285, 206-215, (2010).