Ultrasound-guided microinjection into the mouse forebrain in utero at E9.5

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Pierfelice, Tarran J ^a   Gaiano, Nicholas ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Gene transduction; High frequency ultrasound imaging; Intra ventricular microinjection; Issue 45; Neuroscience; Retroviral vectors; Rodent survival surgery

Indexed keywords

EID: 80355133710     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2047     Document Type: Article

References (2)

1. Yee, J.K. et al. Generation of high-titer pseudotyped retroviral vectors with very broad host range. Methods Cell Biol. 43, 99-112 (1994).
2. Gaiano, N. et al. A method for rapid gain-of-function studies in the mouse embryonic nervous system. Nat Neurosci. 2(9), 812-819 (1999).