Pseudomonas aeruginosa and saccharomyces cerevisiae biofilm in flow cells

Journal of Visualized Experiments

Volumn , Issue 47, 2011, Pages

  Nielsen, Martin Weiss ^a   Sternberg, Claus ^a   Molin, Soren ^a   Regenberg, Birgitte ^b  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355132972     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2383     Document Type: Article

References (15)

1. Costerton, J. W., Stewart, P. S. & Greenberg, E. P. Bacterial biofilms: a common cause of persistent infections. Science 284, 1318-1322 (1999).
2. Sternberg, C. & Tolker-Nielsen, T. Growing and analyzing biofilms in flow cells. Curr Protoc Microbiol Chapter 1, Unit 1B 2, doi:10.1002/9780471729259.mCO1b02s00 (2006).
3. Heydorn, A. et al. Experimental reproducibility in flow-chamber biofilms. Microbiology 146 (Pt 10), 2409-2415 (2000).
4. Pamp, S. J. & Tolker-Nielsen, T. Multiple roles of biosurfactants in structural biofilm development by Pseudomonas aeruginosa. J Bacteriol 189, 2531-2539, doi:10.1128/JB.01515-06 (2007).
5. Haagensen, J. A. et al. Differentiation and distribution of colistin-and sodium dodecyl sulfate-tolerant cells in Pseudomonas aeruginosa biofilms. J Bacteriol 189, 28-37, doi:10.1128/JB.00720-06 (2007).
6. Klausen, M., Aaes-Jorgensen, A., Molin, S. & Tolker-Nielsen, T. Involvement of bacterial migration in the development of complex multicellular structures in Pseudomonas aeruginosa biofilms. Mol Microbiol 50, 61-68, doi:3677 [pii] (2003).
7. Pamp, S. J., Gjermansen, M., Johansen, H. K. & Tolker-Nielsen, T. Tolerance to the antimicrobial peptide colistin in Pseudomonas aeruginosa biofilms is linked to metabolically active cells, and depends on the pmr and mexAB-oprM genes. Mol Microbiol 68, 223-240, doi:10.1111/j.1365-2958.2008.06152.x (2008).
8. Pamp, S. J., Sternberg, C. & Tolker-Nielsen, T. Insight into the microbial multicellular lifestyle via flow-cell technology and confocal microscopy. Cytometry Part A 75A, 90-103 (2009).
9. Barken, K. B. et al. Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms. Environ Microbiol 10, 2331-2343, doi:10.1111/j.1462-2920.2008.01658.x (2008).
10. Qin, Z. et al. Role of autolysin-mediated DNA release in biofilm formation of Staphylococcus epidermidis. Microbiology 153, 2083-2092, doi:10.1099/mic.0.2007/006031-0 (2007).
11. Allesen-Holm, M. et al. A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms. Mol Microbiol 59, 1114-1128, doi:10.1111/j.1365-2958.2005.05008.x (2006).
12. Heydorn, A. et al. Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology 146 (10), 2395-2407 (2000).
13. Vorregaard, M. et al. COMSTAT2, a semi-automated java-based quantification program for the analysis of microbial biofilms. http://www.comstat.dk (2010).
14. Palmer, R. J., Haagensen, J. A., Neu, T. R. & Sternberg, C. in Handbook of Biological Confocal Microscopy (ed James B. Pawley) Ch. 51, 882-900 (Springer, 2006).
15. Haagensen, J. A., Regenberg, B. & Sternberg, C. in High Resolution Microbial Single Cell Analytics Advances in Biochemical Engineering and Biotechnology (eds Susann Müller & Thomas Bley), in press (Springer, 2010).