Ex vivo imaging of T cells in murine lymph node slices with widefield and confocal microscopes

Journal of Visualized Experiments

Volumn , Issue 53, 2011, Pages

  Salmon, Hélène ^a,b   Rivas Caicedo, Ana ^a,b   Asperti Boursin, François ^a,b   Lebugle, Camille ^a,b   Bourdoncle, Pierre ^a,b   Donnadieu, Emmanuel ^a,b  

^a INSTITUT COCHIN   (France)

^b INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355132736     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3054     Document Type: Article

References (6)

1. Asperti-Boursin, F. et al., CCR7 ligands control basal T cell motility within lymph node slices in a phosphoinositide 3-kinase-independent manner. J Exp Med. 204 (5), 1167 (2007).
2. Bhakta, N. R., Oh, D. Y., & Lewis, R. S., Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment. Nat Immunol. 6 (2), 143 (2005).
3. Matheu, M. P., & Cahalan, M. D., Isolation of CD4+ T cells from mouse lymph nodes using Miltenyi MACS purification. J Vis Exp. 9, 409 (2007).
4. Miller, M. J., Wei, S. H., Parker, I., & Cahalan, M. D., Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science. 296 (5574), 1869 (2002).
5. Ehrlich, L. I., Oh, D. Y., Weissman, I. L., & Lewis, R. S., Differential contribution of chemotaxis and substrate restriction to segregation of immature and mature thymocytes. Immunity. 31 (6), 986 (2009).
6. Gollmer, K. et al., CCL21 mediates CD4+ T-cell costimulation via a DOCK2/Rac-dependent pathway. Blood. 114 (3), 580 (2009).