Cell-based calcium assay for medium to high throughput screening of TRP channel functions using FlexStation 3

Journal of Visualized Experiments

Volumn , Issue 54, 2011, Pages

  Luo, Jialie ^a   Zhu, Yingmin ^a   Zhu, Michael X ^a   Hu, Hongzhen ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355132624     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3149     Document Type: Article

References (16)

1. Hu, H., et al. Activation of TRPA1 channels by fenamate nonsteroidal anti-inflammatory drugs. Pflugers Arch. 459 (4), 579-592 (2010).
2. Hu, H., Bandell, M., Petrus, M.J., Zhu, M.X., & Patapoutian, A. Zinc activates damage-sensing TRPA1 ion channels. Nat Chem Biol. 5 (3), 183-190 (2009).
3. Perney, T.M., Hirning, L.D., Leeman, S.E., & Miller, R.J. Multiple calcium channels mediate neurotransmitter release from peripheral neurons. Proc Natl Acad Sci U. S. A. 83 (17), 6656-6659 (1986).
4. Mason, S.A. & MacLeod, K.T. Cardiac action potential duration and calcium regulation in males and females. Biochem Biophys Res Commun. 388 (3), 565-570 (2009).
5. Raat, N.J., Wetzels, G.E., & De Mey, J.G. Calcium-contraction relationship in rat mesenteric arterial smooth muscle. Effects of exogenous and neurogenic noradrenaline. Pflugers Arch. 436 (2), 262-269 (1998).
6. Alagarsamy, S. & Johnson, K.M. Voltage-dependent calcium channel involvement in NMDA-induced activation of NOS. Neuroreport. 6 (16), 2250-2254 (1995).
7. Su, L.T., et al. TRPM7 regulates cell adhesion by controlling the calcium-dependent protease calpain. J Biol Chem. 281 (16), 11260-11270 (2006).
8. Dubinsky, J.M. & Rothman, S.M. Intracellular calcium concentrations during chemical hypoxia and excitotoxic neuronal injury. J Neurosci. 11 (8), 2545-2551 (1991).
9. George, J., et al. Sodium channel activation augments NMDA receptor function and promotes neurite outgrowth in immature cerebrocortical neurons. J Neurosci. 29 (10), 3288-3301 (2009).
10. 2+ - and membrane potential-sensitive dyes: advantages and potential problems. J Neurosci Methods. 149 (2), 172-177 (2005).
11. Laude, A.J., et al. Rapid functional assays of recombinant IP3 receptors. Cell Calcium. 38 (1), 45-51 (2005).
12. Marincsak, R., et al. The analgesic drug, tramadol, acts as an agonist of the transient receptor potential vanilloid-1. Anesth Analg. 106 (6), 1890-1896 (2008).
13. Xie, X., et al. Validation of high throughput screening assays against three subtypes of Ca(v)3 T-type channels using molecular and pharmacologic approaches. Assay Drug Dev Technol. 5 (2), 191-203 (2007).
14. Christensen, B.N., Kochukov, M., McNearney, T.A., Taglialatela, G., & Westlund, K.N. Proton-sensing G protein-coupled receptor mobilizes calcium in human synovial cells. Am J Physiol Cell Physiol. 289 (3), C601-608 (2005).
15. Boulay G, et al. Cloning and expression of a novel mammalian homolog of Drosophila transient receptor potential (Trp) involved in calcium entry secondary to activation of receptors coupled by the Gq class of G protein. J Biol Chem. 272 (47), 29672-29680 (1997).
16. Nanda K, et al. Functional screening of adrenergic receptors by measuring intracellular calcium using the FlexStation scanning fluorimeter. Biotechnol J. 4 (3), 417-422 (2009).