Derivation of enriched oligodendrocyte cultures and oligodendrocyte/neuron myelinating co-cultures from post-natal murine tissues

Journal of Visualized Experiments

Volumn , Issue 54, 2011, Pages

  O'Meara, Ryan W ^a,b   Ryan, Scott D ^a   Colognato, Holly ^c   Kothary, Rashmi ^a,b  

^a OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

^c STONY BROOK UNIVERSITY   (United States)

Author keywords

Co culture; Dorsal root ganglion neuron; In vitro; Issue 54; Mouse; Myelination; Neuroscience; Oligodendrocyte; Primary cells

Indexed keywords

EID: 80355132599     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3324     Document Type: Article

References (10)

1. Avellana-Adalid, V., et al. Expansion of rat oligodendrocyte progenitors into proliferative oligospheres that retain differentiation potential. J Neurosci Res. 45 (5), 558-70 (1996).
2. McCarthy, K.D. & de Vellis, J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol. 85 (3), 890-902 (1980).
3. Barres, B.A., et al. Cell death and control of cell survival in the oligodendrocyte lineage. Cell. 70 (1), 31-46 (1992).
4. Chan, J.R., et al. NGF controls axonal receptivity to myelination by Schwann cells or oligodendrocytes. Neuron. 43 (2), 183-91 (2004).
5. Camara, J., et al. Integrin-mediated axoglial interactions initiate myelination in the central nervous system. J Cell Biol. 185 (4), 699-712 (2009).
6. Ishibashi, T., et al. Astrocytes promote myelination in response to electrical impulses. Neuron. 49 (6), 823-32 (2006).
7. Chen, Y., et al. Isolation and culture of rat and mouse oligodendrocyte precursor cells. Nat Protoc. 2 (5), 1044-51 (2007).
8. Pedraza, C.E., et al. Production, characterization, and efficient transfection of highly pure oligodendrocyte precursor cultures from mouse embryonic neural progenitors. Glia. 56 (12), 1339-52 (2008).
9. Lewin, G.R., Ritter, A.M., & Mendell, L.M. On the role of nerve growth factor in the development of myelinated nociceptors. J Neurosci. 12 (5), 1896-905 (1992).
10. Greene, L.A. Quantitative in vitro studies on the nerve growth factor (NGF) requirement of neurons. II. Sensory neurons. Dev Biol. 58 (1), 106-13 (1977).