Protocol for culturing sympathetic neurons from rat superior cervical ganglia (SCG)

Journal of Visualized Experiments

Volumn , Issue 23, 2009, Pages

  Zareen, Neela ^a   Greene, Lloyd A ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

Apoptosis; Issue 23; Neuroscience; NGF; Primary neuronal culture; Programmed cell death; SCG; Sympathetic neurons; Trophic factor

Indexed keywords

EID: 80355131104     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/988     Document Type: Article

References (8)

1. Banker, G. and Goslin, K. Culturing Nerve Cells. Published by MIT Press, 1998.
2. Biswas, SC; Shi, Y; Sproul, A and Greene, LA. Pro-apoptotic Bim Induction in Response to Nerve Growth Factor Deprivation Requires Simultaneous Activation of Three Different Death Signaling Pathways. The Journal of Biological Chemistry. 282 (40). 29368-29374. (2007).
3. Bocchini, V and Angeletti, PU. The Nerve Growth Factor: purification as a 30,000-molecular-weight protein. Proc. Natl. Acad. Sci. USA (64) 787-794. (1969).
4. Dechant, Georg. Chat in the Trophic Web: NGF Activates Ret by Inter-RTK Signaling. Neuron. 33(2) 156-158 (2002).
5. Deckwerth, T; Elliott, JL; Knudson, CM; Johnson, EM; Snider, WD and Korsmeyer, SJ. Bax is required for neuronal death after trophic factor deprivation and during development. Neuron. (17). 401-411 (1996).
6. Greene, LA. Quantitative in Vitro Studies on the Nerve Growth Factor (NGF) Requirement of Neurons - I. Sympathetic Neurons. Developmental Biology (58). 96-105 (1977).
7. Park, DS; Morris, EJ; Padmanabhan, J; Shelanski, ML; Geller, HM and Greene, LA. Cyclin-dependent kinases participate in death of neurons evoked by DNA-damaging agents. The Journal of Cell Biology. 143, No 2. 457-467 (1998).
8. Pierchala, BA; Ahrens, RC; Paden, AJ and Johnson Jr., EM. Nerve Growth Factor Promotes the Survival of Sympathetic Neurons through the Cooperative Function of the Protein Kinase C and Phosphatidylinositol 3-Kinase Pathways. The Journal of Cell Biology. 279, No 27. 27986-27993 (2004).