Identification of protein complexes with quantitative proteomics in S. cerevisiae

Journal of Visualized Experiments

Volumn , Issue 25, 2009, Pages

  Chao, Jesse Tzu Cheng ^a   Foster, Leonard J ^a   Loewen, Christopher J R ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Amino acid labeling; Biochemistry; ER polarization; Isotope labeling; Isotope coded affinity tag; Issue 25; Quantitation; Quantitative proteomics; Saccharomyces cerevisiae; SILAC; Stable isotope

Indexed keywords

EID: 80355130499     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1225     Document Type: Article

References (4)

1. Loewen, C. J. et al. Inheritance of cortical ER in yeast is required for normal septin organization. Jour. Cell Biol. 179: p467-483 (2007).
2. Ong, S., Foster, L. J., Hoog, C. L. and Mann, M. Mass spectrometric-based approaches in quantitative proteomics. Methods 29: p124-130 (2003).
3. Puig, O. et al. The Tandem Affinity Purification (TAP) method: a general procedure of protein complex purification. Methods 24: p218-229 (2001).
4. Amberg, D. C., Burke, D. J., Strathern, J. N. Methods in Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. p123-125 (2005).