A simple and efficient method to isolate macrophages from mixed primary cultures of adult liver cells

Journal of Visualized Experiments

Volumn , Issue 51, 2011, Pages

  Kitani, Hiroshi ^a   Takenouchi, Takato ^a   Sato, Mitsuru ^a   Yoshioka, Miyako ^b   Yamanaka, Noriko ^b  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355128178     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2757     Document Type: Article

References (10)

1. Crispe, I.N. The liver as a lymphoid organ. Annu. Rev. Immunol. 27, 147-163 (2009).
2. Roberts, R.A., et al. Role of the Kupffer cell in mediating hepatic toxicity and carcinogenesis. Toxicol. Sci. 96, 2-15 (2007).
3. Gao, B., Jeong, W.I. & Tian, Z. Liver: An organ with predominant innate immunity. Hepatology 47, 729-736 (2008).
4. Janousek, J., Strmen, E. & Gervais, F. Purification of murine Kupffer cells by centrifugal elutriation. J. Immunol. Methods 164, 109-117 (1993).
5. Olynyk, J.K. & Clarke, S.L. Isolation and primary culture of rat Kupffer cells. J. Gastroenterol. Hepatol. 13, 842-845 (1998).
6. Heuff, G., Meyer, S. & Beelen, R.H. Isolation of rat and human Kupffer cells by a modified enzymatic assay. J. Immunol. Methods 174, 61-65 (1994).
7. Seglen, P.O. Preparation of isolated rat liver cells. Methods Cell Biol. 13, 29-83 (1976).
8. Yoshioka, M., et al. Primary culture and expression of cytokine mRNAs by lipopolysaccharide in bovine Kupffer cells. Vet. Immunol. Immunopathol. 58, 155-163 (1997).
9. Kitani, H., Takenouchi, T., Sato, M., Yoshioka, M. & Yamanaka, N. A novel isolation method for macrophage-like cells from mixed primary cultures of adult rat liver cells. J. Immunol. Methods 360, 47-55 (2010).
10. Gorrell, M.D. Liver fibrosis: the hepatocyte revisited. Hepatology 46, 1659-1661 (2007).