Detection of signaling effector-complexes downstream of BMP4 using in situ PLA, a proximity ligation assay

Journal of Visualized Experiments

Volumn , Issue 49, 2011, Pages

  Thymiakou, Efstathia ^a   Episkopou, Vasso ^a  

^a HAMMERSMITH HOSPITAL   (United Kingdom)

Author keywords

Behavioral endocrinology; Decision making; Issue 49; Neuroscience; Social status; Testosterone

Indexed keywords

EID: 80355128174     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2631     Document Type: Article

References (8)

1. Miyazono, K., Kamiya, Y., and Morikawa, M. Bone morphogenetic protein receptors and signal transduction. J Biochem. 147(1): 35-51.
2. Gustafsdottir, S.M., et al. Proximity ligation assays for sensitive and specific protein analyses. Anal Biochem 345(1): 2-9 (2005).
3. Soderberg, O., et al. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat Methods 3(12): 995-1000 (2006).
4. Soderberg, O., et al. Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay. Methods 45(3): 227-232 (2008).
5. Yu, P.B., et al. Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism. Nat Chem Biol 4(1): 33-41 (2008).
6. Baan, B., et al. In situ proximity ligation detection of c-Jun/AP-1 dimers reveals increased levels of c-Jun/Fra1 complexes in aggressive breast cancer cell lines in vitro and in vivo. Mol Cell Proteomics. 9(9): 1982-1990.
7. Massinen, S., et al. Functional interaction of DYX1C1 with estrogen receptors suggests involvement of hormonal pathways in dyslexia. Hum Mol Genet 18(15): 2802-12 (2009).
8. Thymiakou, E., Zannis, V.I., and Kardassis, D. Physical and functional interactions between liver X receptor/retinoid X receptor and Sp1 modulate the transcriptional induction of the human ATP binding cassette transporter A1 gene by oxysterols and retinoids. Biochemistry 46(41): 11473-11483 (2007).