A method for production of recombinant mCD1d protein in insect cells

Journal of Visualized Experiments

Volumn , Issue 10, 2007, Pages

  Khurana, Archana ^a   Kronenberg, Mitchell ^a  

^a LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY   (United States)

Author keywords

Alphagalcer; Baculovirus expression system; Bira enzymatic biotynlation; Cell biology; High five insect cells; Issue 10; Mcd1d; Multiplicity of infection; Streptavidin pe

Indexed keywords

EID: 80355127127     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/556     Document Type: Article

References (5)

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2. Kinjo, Y., Wu, D., Kim, G., Xing, G. W., Poles, M. A., Ho, D. D., Tsuji, M., Kawahara, K., Wong, C. H., and Kronenberg, M. (2005) Recognition of bacterial glycosphingolipids by natural killer T cells. Nature 434, 520-525
3. Naidenko, O. V., Maher, J. K., Ernst, W. A., Sakai, T., Modlin, R. L., and Kronenberg, M. (1999). Binding and antigen presentation of ceramide-containing glycolipids by soluble mouse and human CD1d molecules. J. Exp. Med. 190, 1069-1080.
4. Porcelli, S. A. (1995). The CD1 family: A third lineage of antigen- presenting molecules. Adv. Immunol. 59, 1-98
5. Sidobre, S., and Kronemberg, M. (2002). CD1 tetramers: A powerful tool for the analysis of glycolipid-reactive T cells. J. Immunol. 169, 1340-1348.