Usefulness of three-channel multiplex real-time PCR and melting curve analysis for simultaneous detection and identification of the Mycobacterium tuberculosis complex and nontuberculous mycobacteria

Journal of Clinical Microbiology

Volumn 49, Issue 11, 2011, Pages 3963-3966

  Hong, Yun Ji ^a   Chung, Young Hoon ^b   Kim, Taek Soo ^a   Song, Sang Hoon ^a,b   Park, Kyoung Un ^a,b   Yim, Jae Joon ^a   Song, Junghan ^a,b   Lee, Jae Ho ^a   Kim, Eui Chong ^a  

^a Seoul National University College of Medicine   (South Korea)

^b Seoul National University Bundang Hospital   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; CONTROLLED STUDY; HIGH RESOLUTION MELTING ANALYSIS; MYCOBACTERIUM ABSCESSUS; MYCOBACTERIUM AVIUM; MYCOBACTERIUM CHELONEI; MYCOBACTERIUM FORTUITUM; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM KANSASII; MYCOBACTERIUM MARINUM; MYCOBACTERIUM TUBERCULOSIS; MYCOBACTERIUM XENOPEI; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; STRAIN DIFFERENCE;

BACTERIOLOGICAL TECHNIQUES; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOBACTERIUM INFECTIONS; MYCOBACTERIUM TUBERCULOSIS; NONTUBERCULOUS MYCOBACTERIA; REAL-TIME POLYMERASE CHAIN REACTION; TRANSITION TEMPERATURE;

CORYNEBACTERINEAE; MYCOBACTERIUM TUBERCULOSIS COMPLEX;

EID: 80355126256     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.05662-11     Document Type: Article

References (17)

1. American Thoracic Society. 1997. Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Am. J. Respir. Crit. Care Med. 156:S1-S25.
2. Bicmen, C., et al. 2011. Molecular detection and identification of Mycobacterium tuberculosis complex and four clinically important nontuberculous mycobacterial species in smear-negative clinical samples by the GenoType Mycobacteria Direct test. J. Clin. Microbiol. 49:2874-2878.
3. Kang, S. H., K. C. Yoo, K. U. Park, J. Song, and E. C. Kim. 2007. Usefulness of multiplex real-time PCR and melting curve analysis in identification of nontuberculous mycobacteria. Korean J. Lab. Med. 27:40-45.
4. Koh, W. J., and O. J. Kwon. 2006. Nontuberculous mycobacterial lung disease. J. Korean Med. Assoc. 49:806-816.
5. Korean Academy of Tuberculosis and Respiratory Diseases. 1995. National survey of mycobacterial diseases other than tuberculosis in Korea. Tuberc. Respir. Dis. 42:277-294.
6. Lachnik, J., et al. 2002. Rapid-cycle PCR and fluorimetry for detection of mycobacteria. J. Clin. Microbiol. 40:3364-3373. (Pubitemid 34977455)
7. Ngan, G. J., L. M. Ng, R. Jureen, R. T. Lin, and J. W. Teo. 2011. Development of multiplex PCR assays based on the 16S-23S rRNA internal transcribed spacer for the detection of clinically relevant nontuberculous mycobacteria. Lett. Appl. Microbiol. 52:546-554.
8. Park, J. S. 2009. Respiratory review of 2009: nontuberculous Mycobacterium. Tuberc. Respir. Dis. 67:395-401.
9. Portaels, F. 1995. Epidemiology of mycobacterial diseases. Clin. Dermatol. 13:207-222.
10. Prince, D. S., et al. 1989. Infection with Mycobacterium avium complex in patients without predisposing conditions. N. Engl. J. Med. 321:863-868.
11. Ringuet, H., et al. 1999. hsp65 sequencing for identification of rapidly growing mycobacteria. J. Clin. Microbiol. 37:852-857. (Pubitemid 29091327)
12. Sedlacek, L., M. Rifai, K. Feldmann, and F. C. Bange. 2004. LightCyclerbased differentiation of Mycobacterium abscessus and Mycobacterium chelonae. J. Clin. Microbiol. 42:3284-3287. (Pubitemid 38938092)
13. Shinnick, T. M. 1987. The 65-kilodalton antigen of Mycobacterium tuberculosis. J. Bacteriol. 169:1080-1088. (Pubitemid 17043371)
14. Springer, B., L. Stockman, K. Teschner, G. D. Roberts, and E. C. Böttger. 1996. Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J. Clin. Microbiol. 34:296-303. (Pubitemid 26035100)
15. Subcommittee of the Joint Tuberculosis Committee of the British Thoracic Society. 2000. Management of opportunist mycobacterial infections: Joint Tuberculosis Committee guidelines 1999. Thorax 55:210-218.
16. Telenti, A., et al. 1993. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J. Clin. Microbiol. 31:175-178. (Pubitemid 23028605)
17. Zakowski, P., S. Fligiel, G. W. Berlin, and L. Johnson, Jr. 1982. Disseminated Mycobacterium avium-intracellulare infection in homosexual men dying of acquired immunodeficiency. JAMA 248:2980-2982. (Pubitemid 13243890)