Whole-cell recording of Calcium Release-Activated Calcium (CRAC) currents in human T lymphocytes

Journal of Visualized Experiments

Volumn , Issue 46, 2010, Pages

  Thakur, Pratima ^a   Fomina, Alla F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

CRAC channels; CRAC currents; Human T lymphocytes; Patch clamp

Indexed keywords

EID: 80355125645     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2346     Document Type: Article

References (10)

1. Parekh, A.B. Store-operated CRAC channels: function in health and disease. Nat Rev Drug Discov 9, 399-410 (2010).
2. Feske, S. CRAC channelopathies. Pflugers Arch (2010).
3. Lewis, R.S. & Cahalan, M.D. Mitogen-induced oscillations of cytosolic Ca2+ and transmembrane Ca2+ current in human leukemic T cells. Cell Regul 1, 99-112 (1989).
4. Zweifach, A. & Lewis, R.S. Rapid inactivation of depletion-activated calcium current (ICRAC) due to local calcium feedback. J Gen Physiol 105, 209-26 (1995).
5. Zweifach, A. & Lewis, R.S. Slow calcium-dependent inactivation of depletion-activated calcium current. Store-dependent and-independent mechanisms. J Biol Chem 270, 14445-51 (1995).
6. Zweifach, A. & Lewis, R.S. Calcium-dependent potentiation of store-operated calcium channels in T lymphocytes. J Gen Physiol 107, 597-610 (1996).
7. Prakriya, M. & Lewis, R.S. Separation and characterization of currents through store-operated CRAC channels and Mg2+-inhibited cation (MIC) channels. J Gen Physiol 119, 487-507 (2002).
8. Fomina, A.F., Fanger, C.M., Kozak, J.A. & Cahalan, M.D. Single channel properties and regulated expression of Ca(2+) release-activated Ca(2+) (CRAC) channels in human T cells. J Cell Biol 150, 1435-44 (2000).
9. Sakmann, B. & Neher, E. Single-Channel Recording (2nd edition), Plenum Press, New York and London. (1995).
10. Neher, E. Correction for liquid junction potentials in patch clamp experiments. Methods Enzymol 207, 123-31 (1992).