Optimized transfection strategy for expression and electrophysiological recording of recombinant voltage-gated ion channels in HEK-293T cells

Journal of Visualized Experiments

Volumn , Issue 47, 2010, Pages

  Senatore, Adriano ^a   Boone, Adrienne N ^a   Spafford, J David ^a  

^a UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Brain; Calcium channel; Electrophysiology; Invertebrate; Issue 47; Neuroscience; Voltage gated

Indexed keywords

EID: 80355125504     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2314     Document Type: Article

References (6)

1. Thomas, P., Smart, T.G. HEK293 cell line: A vehicle for the expression of recombinant proteins. J. Pharmacological and Toxicological Methods 51, 187-200 (2005).
2. Senatore, A., Spafford, J.D. Transient and big are key features of an invertebrate T-type channel (LCav3) from the central nervous system of Lymnaea stagnalis Journal of Biological Chemistry 285, 7447-7458 (2010).
3. Senatore, A., Boone, A. N., Lam, S., Dawson, T. F., Zhorov, B. S., Spafford, J. D. "Mapping of dihydropyridine (isradipine) binding residues in a less sensitive invertebrate L-type calcium channel (LCav1)". (submitted).
4. nd edn. The Company of Biologists, Cambridge (1987).
5. Molleman, A. Patch clamping: an introductory guide to patch clamp electrophysiology. John Wiley & Sons Ltd., England (2003).
6. 2+channels. Brain Research 1045, 116-123 (2005).