Co-expression of multiple target proteins in plants from a tobacco mosaic virus vector using a combination of homologous and heterologous subgenomic promoters

Archives of Virology

Volumn 156, Issue 11, 2011, Pages 2057-2061

  Roy, Gourgopal ^a,b   Weisburg, Sangeetha ^a   Foy, Kelly ^a   Rabindran, Shailaja ^a,c   Mett, Vadim ^a   Yusibov, Vidadi ^a  

^a Fraunhofer USA Inc   (United States)

^b DSM RESOLVE   (Netherlands)

^c UNITED STATES DEPARTMENT OF AGRICULTURE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAPSID PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; PROMOTER REGION; REPORTER GENE; TOBACCO; TOBACCO MOSAIC VIRUS; VIROLOGY;

CAPSID PROTEINS; GENE EXPRESSION; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; TOBACCO; TOBACCO MOSAIC VIRUS;

AVIAN INFLUENZA VIRUS; MIRIDAE; NICOTIANA BENTHAMIANA; TOBACCO MOSAIC VIRUS;

EID: 80255135938     PISSN: 03048608     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00705-011-1069-x     Document Type: Article

References (16)

1. Dawson WO, Lewandowski DJ, Hilf ME, Bubrick P, Raffo AJ, Shaw JJ, Grantham GL, Desjardins PR (1989) A tobacco mosaic virus-hybrid expresses and loses an added gene. Virology 172: 285-292.
2. Giritch A, Marillonnet S, Engler C, van Eldik G, Botterman J, Klimyuk V, Gleba Y (2006) Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors. Proc Natl Acad Sci USA 103: 14701-14706.
3. Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM (2000) pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Mol Biol 42: 819-832.
4. Kumar D, Klessig DF (2003) High-affinity salicylic acid-binding protein 2 is required for plant innate immunity and has salicylic acid-stimulated lipase activity. Proc Natl Acad Sci USA 100: 16101-16106.
5. Lindbo JA (2007) TRBO: a high-efficiency tobacco mosaic virus RNA-based overexpression vector. Plant Physiol 145: 1232-1240.
6. Man M, Epel BL (2004) Characterization of regulatory elements within the coat protein (CP) coding region of Tobacco mosaic virus affecting subgenomic transcription and green fluorescent protein expression from the CP subgenomic RNA promoter. J Gen Virol 85: 1727-1738.
7. Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat Biotechnol 23: 718-723.
8. Park SW, Kaimoyo E, Kumar D, Mosher S, Klessig DF (2007) Methyl salicylate is a critical mobile signal for plant systemic acquired resistance. Science 318: 113-116.
9. Roy G, Weisburg S, Rabindran S, Yusibov V (2010) A novel two-component Tobacco mosaic virus-based vector system for high-level expression of multiple therapeutic proteins including a human monoclonal antibody in plants. Virology 405: 93-99.
10. Shivprasad S, Pogue GP, Lewandowski DJ, Hidalgo J, Donson J, Grill LK, Dawson WO (1999) Heterologous sequences greatly affect foreign gene expression in tobacco mosaic virus-based vectors. Virology 255: 312-323.
11. Shoji Y, Bi H, Musiychuk K, Rhee A, Horsey A, Roy G, Green B, Shamloul M, Farrance CE, Taggart B, Mytle N, Ugulava N, Rabindran S, Mett V, Chichester JA, Yusibov V (2009) Plant-derived hemagglutinin protects ferrets against challenge infection with the A/Indonesia/05/05 strain of avian influenza. Vaccine 27: 1087-1092.
12. Silhavy D, Molnar A, Lucioli A, Szittya G, Hornyik C, Tavazza M, Burgyan J (2002) A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs. EMBO J 21: 3070-3080.
13. Vlot AC, Dempsey DA, Klessig DF (2009) Salicylic acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol 47: 177-206.
14. Voinnet O, Rivas S, Mestre P, Baulcombe D (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33: 949-956.
15. Yuan ZC, Edlind MP, Liu P, Saenkham P, Banta LM, Wise AA, Ronzone E, Binns AN, Kerr K, Nester EW (2007) The plant signal salicylic acid shuts down expression of the vir regulon and activates quormone-quenching genes in Agrobacterium. Proc Natl Acad Sci USA 104: 11790-11795.
16. Yusibov V, Rabindran S (2008) Recent progress in the development of plant derived vaccines. Expert Rev Vaccines 7: 1173-1183.