Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12

Microbial Cell Factories

Volumn 10, Issue , 2011, Pages

  Ramli, Aizi N M ^a   Mahadi, Nor M ^b   Rabu, Amir ^c   Murad, Abdul M A ^c   Bakar, Farah D A ^c   Illias, Rosli M ^a  

^a UNIVERSITI TEKNOLOGI MALAYSIA   (Malaysia)

^b MALAYSIA GENOME INSTITUTE   (Malaysia)

^c UNIVERSITI KEBANGSAAN MALAYSIA   (Malaysia)

Author keywords

Cold adapted chitinase; Glaciozyma antarctica; Pi12. psychrophilic yeast; Pichia pastoris

Indexed keywords

CHITIN; CHITINASE; GLYCOL; RECOMBINANT ENZYME; FUNGAL PROTEIN; RECOMBINANT PROTEIN;

ACIDITY; ARTICLE; BINDING AFFINITY; BIOTECHNOLOGY; COLD ACCLIMATIZATION; COLLOID; ENZYME STABILITY; FOOD SPOILAGE; GENE ISOLATION; GLACIOZYMA ANTARCTICA; LOW TEMPERATURE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PSYCHROPHILIC BACTERIUM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; AMINO ACID SEQUENCE; BASIDIOMYCETES; CHEMISTRY; CLASSIFICATION; COLD; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PHYLOGENY; PICHIA; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT;

PICHIA PASTORIS;

AMINO ACID SEQUENCE; BASIDIOMYCOTA; CHITINASE; CLONING, MOLECULAR; COLD TEMPERATURE; ENZYME STABILITY; FUNGAL PROTEINS; GENE EXPRESSION; KINETICS; MOLECULAR SEQUENCE DATA; PHYLOGENY; PICHIA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY;

EID: 80155123418     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-10-94     Document Type: Article

References (58)

1. Bae E, Phillips GN. Structures and Analysis of Highly Homologous Psychrophilic, Mesophilic, and Thermophilic Adenylate Kinases. Journal of Biological Chemistry 2004, 279:28202-28208.
2. Cavicchioli R, Siddiqui KS, Andrews D, Sowers KR. Low-temperature extremophiles and their applications. Current Opinion in Biotechnology 2002, 13:253-261.
3. Collins T, Meuwis M-A, Gerday C, Feller G. Activity, Stability and Flexibility in Glycosidases Adapted to Extreme Thermal Environments. Journal of Molecular Biology 2003, 328:419-428.
4. Joseph B, Ramteke PW, Thomas G. Cold active microbial lipases: Some hot issues and recent developments. Biotechnology Advances 2008, 26:457-470.
5. Feller G, Lonhienne T, Deroanne C, Libioulle C, Van Beeumen J, Gerday C. Purification, characterization, and nucleotide sequence of the thermolabile alpha-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23. Journal of Biological Chemistry 1992, 267:5217-5221.
6. Lonhienne T, Mavromatis K, Vorgias CE, Buchon L, Gerday C, Bouriotis V. Cloning, Sequences, and Characterization of Two Chitinase Genes from the Antarctic Arthrobacter sp. Strain TAD20: Isolation and Partial Characterization of the Enzymes. The Journal of Bacteriology 2001, 183:1773-1779.
7. Stefanidi E, Vorgias C. Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles 2008, 12:541-552.
8. Kubicek CP, Mach RL, Peterbauer CK, Lorito M. Trichoderma: From genes to biocontrol. Journal of Plant Pathology 2001, 83:11-23.
9. Dahiya N, Tewari R, Tiwari RP, Hoondal GS. Chitinase from Enterobacter sp. NRG4: Its purification, characterization and reaction pattern. Electronic Journal of Biotechnology 2005, 8:134-145.
10. Gooday GW. Physiology of microbial degradation of chitin and chitosan. Biodegradation 1990, 1:177-190.
11. Patil RS, Ghormade V, Deshpande MV. Chitinolytic enzymes: an exploration. Enzyme and Microbial Technology 2000, 26:473-483.
12. Dahiya N, Tewari R, Hoondal G. Biotechnological aspects of chitinolytic enzymes: a review. Applied Microbiology and Biotechnology 2006, 71:773-782.
13. Xiao X, Yin X, Lin J, Sun L, You Z, Wang P, Wang F. Chitinase genes in lake sediments of Ardley Island, Antarctica. Applied and Environmental Microbiology 2005, 71:7904-7909.
14. McCreath KJ, Specht CA, Robbins PW. Molecular cloning and characterization of chitinase genes from Candida albicans. Proceedings of the National Academy of Sciences of the United States of America 1995, 92:2544-2548.
15. Henrissat B, Bairoch A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochemical Journal 1993, 293:781-788.
16. Tronelli D, Maugini E, Bossa F, Pascarella S. Structural adaptation to low temperatures - analysis of the subunit interface of oligomeric psychrophilic enzymes. FEBS Journal 2007, 274:4595-4608.
17. Orikoshi H, Baba N, Nakayama S, Kashu H, Miyamoto K, Yasuda M, Inamori Y, Tsujibo H. Molecular analysis of the gene encoding a novel cold-adapted chitinase (ChiB) from a marine bacterium, Alteromonas sp. Strain O-7. The Journal of Bacteriology 2003, 185:1153-1160.
18. Nakamura T, Ishikawa M, Nakatani H, Oda A. Characterization of cold-responsive extracellular chitinase in bromegrass cell cultures and its relationship to antifreeze activity. Plant Physiology 2008, 147:391-401.
19. Fenice M, Selbmann L, Di Giambattista R, Federici F. Chitinolytic activity at low temperature of an Antarctic strain (A3) of Verticillium lecanii. Research in Microbiology 1998, 149:289-300.
20. Papa R, Rippa V, Sannia G, Marino G, Duilio A. Recombinant protein expression system in cold loving microorganisms. Microbial Cell Factories 2006, 5:1-2.
21. Feller G, Thiry M, Arpigny JL, Gerday C. Cloning and expression in Escherichia coli of three lipase-encoding genes from the psychrotrophic antarctic strain Moraxella TA144. Gene 1991, 102:111-115.
22. Jahic M, Wallberg F, Bollok M, Garcia P, Enfors S-O. Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microbial Cell Factories 2003, 2:6.
23. Gan Z, Yang J, Tao N, yu Z, Zhang K-Q. Cloning and expression analysis of a chitinase gene Crchi1 from the mycoparasite fungus Clonostachys rosea (syn. Gliocladium roseum). The Journal of Mirobiology 2007, 45:422-430.
24. Wang Y-J, Yang Q. Cloning and Expression of a Novel Chitinase chi58 from Chaetomium cupreum in Pichia pastoris. Biochemical Genetics 2009, 47:547-558.
25. Bendt A, Hüller H, Kammel U, Helmke E, Schweder T. Cloning, expression, and characterization of a chitinase gene from the Antarctic psychrotolerant bacterium Vibrio sp. strain Fi:7. Extremophiles 2001, 5:119-126.
26. Dyrløv Bendtsen J, Nielsen H, von Heijne G, Brunak S. Improved Prediction of Signal Peptides: SignalP 3.0. Journal of Molecular Biology 2004, 340:783-795.
27. Gupta R, Brunak S. Prediction of glycosylation across the human proteome and the correlation to protein function. Pacific Symposium on Biocomputing 2002, 310-322.
28. Hunter S, Apweiler R, Attwood TK, Bairoch A, Bateman A, Binns D, Bork P, Das U, Daugherty L, Duquenne L, et al. InterPro: the integrative protein signature database. Nucleic Acids Research 2009, 37:D211-D215.
29. Wang S-Y, Wu S-J, Thottappilly G, Locy RD, Singh NK. Molecular cloning and structural analysis of the gene encoding Bacillus cereus exochitinase Chi36. Journal of Bioscience and Bioengineering 2001, 92:59-66.
30. Limón MC, Chacón MR, Mejías R, Delgado-Jarana J, Rincón AM, Codón AC, Benítez T. Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain. Applied Microbiology and Biotechnology 2004, 64:675-685.
31. Liu ZH, Yang Q, Hu S, Zhang JD, Ma J. Cloning and characterization of a novel chitinase gene (chi46) from Chaetomium globosum and identification of its biological activity. Applied Microbiology and Biotechnology 2008, 80:241-252.
32. Gan Z, Yang J, Tao N, Liang L, Mi Q, Li J, Zhang K-Q. Cloning of the gene Lecanicillium psalliotae chitinase Lpchi1 and identification of its potential role in the biocontrol of root-knot nematode Meloidogyne incognita. Applied Microbiology and Biotechnology 2007, 76:1309-1317.
33. Kawase T, Saito A, Sato T, Kanai R, Fujii T, Nikaidou N, Miyashita K, Watanabe T. Distribution and phylogenetic analysis of family 19 chitinases in Actinobacteria. Appl Environ Microbiol 2004, 70:1135-1144.
34. Mattanovich D, Gasser B, Hohenblum H, Sauer M. Stress in recombinant protein producing yeasts. Journal of Biotechnology 2004, 113:121-135.
35. Hong F, Meinander NQ, Jönsson LJ. Fermentation strategies for improved heterologous expression of laccase in Pichia pastoris. Biotechnology and Bioengineering 2002, 79:438-449.
36. Lee SG, Koh HY, Han SJ, Park H, Na DC, Kim I-C, Lee HK, Yim JH. Expression of recombinant endochitinase from the Antarctic bacterium, Sanguibacter antarcticus KOPRI 21702 in Pichia pastoris by codon optimization. Protein Expression and Purification 2010, 71:108-114.
37. Julenius K, MÃ̧lgaard A, Gupta R, Brunak S. Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites. Glycobiology 2005, 15:153-164.
38. You M, Xuan X, Tsuji N, Kamio T, Taylor D, Suzuki N, Fujisaki K. Identification and molecular characterization of a chitinase from the hard tick Haemaphysalis longicornis. Journal of Biological Chemistry 2003, 278:8556-8563.
39. Park S-M, Kim D-H, Truong NH, Itoh Y. Heterologous expression and characterization of class III chitinases from rice (Oryza sativa L.). Enzyme and Microbial Technology 2002, 30:697-702.
40. El-Katatny MH, Gudelj M, Robra KH, Elnaghy MA, Gubitz GM. Characterization of a chitinase and an endo-beta-1,3-glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii. Applied Microbiology and Biotechnology 2001, 56:137-143.
41. Chen WM, Chen GH, Chen CS, Jiang ST. Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS Expression System. Bioscience, Biotechnology and Biochemistry 2009, 73:1172-1174.
42. Alias N, Mahadi N, Murad A, Bakar F, Mahmood N, Illias R. Expression and characterization of Trichoderma virens UKM-1 endochitinase in Escherichia coli. World Journal of Microbiology and Biotechnology 2009, 25:561-572.
43. Sandhya C, Adapa LK, Nampoothiri KM, Binod P, Szakacs G, Pandey A. Extracellular chitinase production by Trichoderma harzianum in submerged fermentation. Journal of Basic Microbiology 2004, 44:49-58.
44. Yano S, Rattanakit N, Wakayama M, Tachiki T. A chitinase indispensable for formation of protoplast of Schizophyllum commune in basidiomycete-lytic enzyme preparation produced by Bacillus circulans KA-304. Bioscience, Biotechnology and Biochemistry 2004, 68:1299-1305.
45. Bhushan B, Hoondal GS. Isolation, purification and properties of a thermostable chitinase from an alkalophilic Bacillus sp. BG-11. Biotechnology Letters 1998, 20:157-159.
46. Woo C-J, Park H-D. An extracellular Bacillus sp. chitinase for the production of chitotriose as a major chitinolytic product. Biotechnology Letters 2003, 25:409-412.
47. Siddiqui KS, Cavicchioli R. Cold-adapted enzymes. Annual Review of Biochemistry 2006, 75:403-433.
48. Feller G. Molecular adaptations to cold in psychrophilic enzymes. Cellular and Molecular Life Sciences 2003, 60:648-662.
49. Fields PA, Somero GN. Hot spots in cold adaptation: Localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes. Proceedings of the National Academy of Sciences 1998, 95:11476-11481.
50. D'Amico S, Gerday C, Feller G. Structural determinants of cold adaptation and stability in a psychrophilic α-amylase. Biologia (Bratislava) 2002, 57:213-219.
51. Sokolovsky VY, Kaldenhoff R, Ricci M, Russo VEA. Fast and reliable mini-prep RNA extraction from Neurospora crassa. Fungal Genetics Newsletters 1990, 37:39-40.
52. Thompson JD, Gibson TJ, Higgins DG. Multiple Sequence Alignment Using ClustalW and ClustalX. Current Protocols in Bioinformatics 2002, John Wiley & Sons, Inc.
53. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 1959, 31:426-428.
54. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976, 72:248-254.
55. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
56. Kuranda MJ, Robbins PW. Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. Journal of Biological Chemistry 1991, 266:19758-19767.
57. Fitches E, Wilkinson H, Bell H, Bown DP, Gatehouse JA, Edwards JP. Cloning, expression and functional characterisation of chitinase from larvae of tomato moth (Lacanobia oleracea): a demonstration of the insecticidal activity of insect chitinase. Insect Biochemistry and Molecular Biology 2004, 34:1037-1050.
58. Watanabe T, Oyanagi W, Suzuki K, Ohnishi K, Tanaka H. Structure of the gene encoding chitinase D of Bacillus circulans WL-12 and possible homology of the enzyme to other prokaryotic chitinases and class III plant chitinases. J Bacteriol 1992, 174:408-414.