Chromosomal spread preparation of human embryonic stem cells for karyotyping

Journal of Visualized Experiments

Volumn , Issue 31, 2009, Pages

  Campos, Priscila B ^a   Sartore, Rafaela C ^a   Abdalla, Stacie N ^a   Rehen, Stevens K ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Aneuploidy; Cellular Biology; Chromosome spreads; Cytogenetics; Human embryonic stem cells; Issue 31

Indexed keywords

EID: 80055122094     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1512     Document Type: Article

References (10)

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3. Maitra, A. et al., Genomic alterations in cultured human embryonic stem cells. Nat Genet 37 (10), 1099 (2005).
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5. Buzzard, J. J., Gough, N. M., Crook, J. M., and Colman, A., Karyotype of human ES cells during extended culture. Nat Biotechnol 22 (4), 381 (2004).
6. Caisander, G. et al., Chromosomal integrity maintained in five human embryonic stem cell lines after prolonged in vitro culture. Chromosome Res 14 (2), 131 (2006).
7. Imreh, M. P. et al., In vitro culture conditions favoring selection of chromosomal abnormalities in human ES cells. J Cell Biochem 99 (2), 508 (2006).
8. Robin-Wesselschmidt, Jeanne Loring;, in Human Stem Cell Manual, edited by Jeanne F Loring; Robin L Wesselschmidt and Philip H Schwartz (Elsevier{replacement character}s Science & Technology Rights, California 2007), pp. 59.
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10. Heslop-Harrison, Trude Schwarzacher;, in Practical in situ hybridization, edited by Trude Schwarzacher and Heslop-Harrison (BIOS Scientific Publishers, Oxford, 2000), pp. 51.