Fast and sensitive colloidal Coomassie G-250 staining for proteins in polyacrylamide gels

Journal of Visualized Experiments

Volumn , Issue 30, 2009, Pages

  Dyballa, Nadine ^a   Metzger, Sabine ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055120991     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1431     Document Type: Article

References (5)

1. Fazekas de St Groth, S., Webster, R. G. & Datyner, A., Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. Biochim. Biophys. Acta 71, 377-391 (1963).
2. Neuhoff, V., Arold, N., Taube, D. & Ehrhardt, W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9, 255-262 (1988).
3. Candiano, G., Bruschi, M., Musante, L., Santucci, L., Ghiggeri, G.M., Carnemolla, B., Orecchia, P., Zardi, L. & Rigetti, P.G. Blue Silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25, 1327-1333 (2004).
4. Kang, D., Gho, S.G., Suh, M. & Kang, C. Highly Sensitive and Fast Protein Detection with Coomassie Brilliant Blue in Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Bull. Korean Chem. Soc. 11, 1511-1512 (2002).
5. Shevchenko, A., Wilm, M., Vorm, O. & Mann, M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68, 850-858 (1996).