Standardized preparation of single-cell suspensions from mouse lung tissue using the gentleMACS dissociator

Journal of Visualized Experiments

Volumn , Issue 29, 2009, Pages

  Jungblut, Melanie ^a   Oeltze, Karen ^a   Zehnter, Irene ^a   Hasselmann, Doris ^a   Bosio, Andreas ^a  

^a MILTENYI BIOTEC GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055120743     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1266     Document Type: Article

References (2)

1. Swanson, K., et al. Flt3-Ligand, IL-4, GM-CSF, and Adherence-Mediated Isolation of Murine Lung Dendritic Cells: Assessment of Isolation Technique on Phenotype and Function. J. Immunol. 173: 4875-4881 (2004).
2. Vermaelen, K., and Pauwels, R. Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by flow cytometry: Methodology and new insights. Cytometry Part A 61A: 170-177 (2004).