Selective labelling of cell-surface proteins using CyDye DIGE Fluor minimal Dyes

Journal of Visualized Experiments

Volumn , Issue 21, 2008, Pages

  Hagner Mcwhirter, Asa ^a   Winkvist, Maria ^a   Bourin, Stephanie ^a   Marouga, Rita ^a  

^a GE Healthcare Bio Sciences AB   (Sweden)

Author keywords

2 D electrophoresis; Biochemistry; Cell surface protein labelling; Cell surface proteins; CyDYE DIGE fluor minimal dyes; Ettan DIGE; Fluorescence; Issue 21; Receptors

Indexed keywords

EID: 80055120646     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/945     Document Type: Article

References (8)

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2. Shin, B.K. et al. Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function. J Biol Chem. 9, 7607-16 (2003).
3. Ettan DIGE System User Manual, GE Healthcare user manual 18-1164-40 (2006).
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5. 2-D Electrophoresis using immobilized pH gradients, principles and methods. GE Healthcare handbook 80-6429-60 Edition AB (2002).
6. CyDye DIGE fluors and labeling kits. GE Healthcare data file 18-1164-84 Edition AB (2003).
7. Mayrhofer, C., Krieger, S, Allmaier, G and Kerjaschki, D. DIGE compatible labeling of surface proteins on vital cells in vitro and in vivo. Proteomics 2, 579-85 (2006).
8. Selective labeling of cell-surface proteins using CyDye DIGE Fluor minimal dyes. GE Healthcare Application Note 11-0033-92 Edition AB (2005).