Microinjection of xenopus laevis oocytes

Journal of Visualized Experiments

Volumn , Issue 24, 2009, Pages

  Cohen, Sarah ^a   Au, Shelly ^a   Panté, Nelly ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Cellular biology; Electron microscopy; Issue 24; Microinjection; Nuclear import; Nuclear import of viruses; Nuclear membrane; Nuclear pore complex; Xenopus oocyte

Indexed keywords

EID: 80055119759     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1106     Document Type: Review

References (5)

1. Panté, N. Use of intact Xenopus oocytes in nucleocytoplasmic transport studies. Methods Mol. Biol. 322, 301-314 (2006).
2. Panté, N. & Kann, M. Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm. Mol. Biol. Cell 13(2), 425-434 (2002).
3. Rabe, B., Vlachou, A., Panté, N., Helenius, A., & Kann, M. Nuclear import of hepatitis B virus capsids and release of the viral genome. Proc. Natl. Acad. Sci. U.S.A. 100(17), 9849-9854 (2003).
4. Cohen, S. & Panté, N. Pushing the Envelope: Microinjection of MVM into Xenopus oocytes causes damage to the nuclear envelope. J. Gen. Virol. 86(12), 3243-3252 (2005).
5. Panté, N., Jarmolowski, A., Izaurralde, E., Sauder, U., Baschong, W. & Mattaj, I. W. Visualizing nuclear export of different classes of RNA by electron microscopy. RNA 3, 498-513 (1997).