Deciphering axonal pathways of genetically defined groups of neurons in the chick neural tube utilizing in ovo electroporation

Journal of Visualized Experiments

Volumn , Issue 39, 2010, Pages

  Avraham, Oshri ^a   Zisman, Sophie ^a   Hadas, Yoav ^a   Vald, Lilach ^a   Klar, Avihu ^a  

^a HEBREW UNIVERSITY   (Israel)

Author keywords

Axonal pathway; Chick; In ovo electroporation; Interneurons; Issue 39; Neural tube; Neuroscience

Indexed keywords

EID: 80055117638     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1792     Document Type: Article

References (5)

1. Zisman, S. et al., Proteolysis and membrane capture of F-spondin generates combinatorial guidance cues from a single molecule. J Cell Biol 178 (7), 1237-1249 (2007).
2. Arakawa, T., Iwashita, M., Matsuzaki, F., Suzuki, T., & Yamamoto, T., Paths, elongation, and projections of ascending chick embryonic spinal commissural neurons after crossing the floor plate. Brain Res 1223, 25-33 (2008).
3. Avraham, O. et al., Transcriptional control of axonal guidance and sorting in dorsal interneurons by the Lim-HD proteins Lhx9 and Lhx1. Neural Dev 4 (1), 21 (2009).
4. Timmer, J., Johnson, J., & Niswander, L., The use of in ovo electroporation for the rapid analysis of neural-specific murine enhancers. Genesis 29 (3), 123-132 (2001).
5. Reeber, S.L. et al., Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord. J Neurosci 28 (35), 8698-8708 (2008).