The importance of correct protein concentration for kinetics and affinity determination in structure-function analysis

Journal of Visualized Experiments

Volumn , Issue 37, 2010, Pages

  Pol, Ewa ^a  

^a BIACORE AB   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055114484     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1746     Document Type: Article

References (6)

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3. Stubbs, M. T., Laber, B., Bode, W., Huber, R., Jerala, R., Lenarcic, B. & Turk, V. The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction. EMBO J. 9 (6), 1939-1947 (1990).
4. Pol, E. & Björk, I. Importance of the second binding loop and the C-terminal end of cystatin B (Stefin B) for inhibition of cysteine proteinases. Biochemistry 38 (32), 10519-10526 (1999).
5. Björk, I., Pol, E., Raub-Segall, E., Abrahamson, M., Rowan, A. D. & Mort, J. S. Differential changes in the association and dissociation rate constants for binding of cystatins to target proteinases occurring on N-terminal truncation of the inhibitors indicate that the interaction mechanism varies with different enzymes. Biochem. J. 299 (Pt 1), 219-225 (1994).
6. Pace, C.N., Vajdos, F., Fee, L., Grimsley, G. & Gray, T. How to measure and predict the molar absorption coefficient of a protein. ProteinScience, 4(11), 2411-2423 (1995).