Measuring near plasma membrane and global intracellular calcium dynamics in astrocytes

Journal of Visualized Experiments

Volumn , Issue 26, 2009, Pages

  Shigetomi, Eiji ^a   Khakh, Baljit S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Astrocyte; Brain; Calcium imaging; Culture; Hippocampus; Issue 26; Neuroscience; Rat; TIRF

Indexed keywords

EID: 80055114266     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1142     Document Type: Article

References (15)

1. Richler, E., Chaumont, S., Shigetomi, E., Sagasti, A. & Khakh, B. S. An approach to image activation of transmitter-gated P2X receptors in vitro and in vivo. Nature Methods 5, 87-93 (2008).
2. Granseth, B., Odermatt, B., Royle, S. J. & Lagnado, L. Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses. Neuron 51, 773-86 (2006).
3. Nunez J. Primary culture of hippocampal neurons from P0 newborn rats. J Vis Exp 19 (2008)
4. Steyer, J. A. & Almers, W. A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol 2, 268-75 (2001).
5. Jaiswal, J. K., Fix, M., Takano, T., Nedergaard, M. & Simon, S. M. Resolving vesicle fusion from lysis to monitor calcium-triggered lysosomal exocytosis in astrocytes. Proc Natl Acad Sci U S A 104, 14151-6 (2007).
6. Araque, A., Carmignoto, G. & Haydon, P. G. Dynamic signalling between astrocytes and neurons. Annu Rev Physiol 63, 795-813 (2001).
7. Haydon, P. G. GLIA: listening and talking to the synapse. Nat Rev Neurosci 2, 185-93 (2001).
8. Bowser, D. N. & Khakh, B. S. ATP excites interneurons and astrocytes to increase synaptic inhibition in neuronal networks. J Neurosci 24, 8606-20 (2004).
9. Bowser, D. N. & Khakh, B. S. Two forms of astrocyte single vesicle exocytosis imaged with total internal reflection fluorescence microscopy. Proc Natl Acad Sci U S A 104, 4212-7 (2007).
10. Bowser, D. N. & Khakh, B. S. Vesicular ATP is the predominant cause of intercellular calcium waves in astrocytes. J Gen Physiol 129, 485-91 (2007).
11. Agulhon, C. et al. What is the role of astrocyte calcium in neurophysiology? Neuron 59, 932-46 (2008).
12. Barres, B. A. The mystery and magic of glia: a perspective on their roles in health and disease. Neuron 60, 430-40 (2008).
13. Lee, S. Y. & Haydon, P. G. Astrocytic glutamate targets NMDA receptors. J Physiol 581, 887-8 (2007).
14. Shigetomi, E., Bowser, D. N., Sofroniew, M. V. & Khakh, B. S. Two forms of astrocyte calcium excitability have distinct effects on NMDA receptor-mediated slow inward currents in pyramidal neurons. J Neurosci 28, 6659-63 (2008).
15. Cahoy, J. D. et al. A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function. J Neurosci 28, 264-78 (2008).