Live-imaging of PKC translocation in sf9 cells and in aplysia sensory neurons

Journal of Visualized Experiments

Volumn , Issue 50, 2011, Pages

  Farah, Carole A ^a   Sossin, Wayne S ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

Aplysia; Confocal microscopy; Issue 50; Live imaging; Microinjection of plasmid dna; Neurons; Neuroscience; Pkc; Sf9 cells; Translocation

Indexed keywords

EID: 80055113393     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2516     Document Type: Article

References (5)

1. Farah, C. A., Nagakura, I., Weatherill, D., Fan, X. & Sossin, W. S. Physiological role for phosphatidic acid in the translocation of the novel protein kinase C Apl II in Aplysia neurons. Mol Cell Biol 28, 4719-4733 (2008).
2. 2+-dependent PKC APL I in the induction of intermediate-term facilitation. J Neurosci 26, 8847-8856 (2006).
3. Farah, C. A., Weatherill, D., Dunn, T. W. & Sossin, W. S. PKC differentially translocates during spaced and massed training in Aplysia. J Neurosci 29, 10281-10286, (2009).
4. Zhao, Y., Wang, D. O. & Martin, K. C. Preparation of Aplysia sensory-motor neuronal cell cultures. J Vis Exp, doi:1355 [pii]10.3791/1355 (2009).
5. 2+-independent PKC Apl II mediates the serotonin induced facilitation at depressed synapses in Aplysia. J. Neuroscience 21, 1247-1256 (2001).