Dissection and culture of commissural neurons from embryonic spinal cord

Journal of Visualized Experiments

Volumn , Issue 39, 2010, Pages

  Langlois, Sébastien D ^a,b   Morin, Steves ^a   Yam, Patricia T ^a,b,c   Charron, Frédéric ^a,b,d  

^a CLIN RESEARCH INSTITUTE OF MONTREAL   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c MCGILL UNIVERSITY   (Canada)

^d UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Axon guidance; Commissural neurons; Development; Dissection; Issue 39; Jove neuroscience; Primary neuron culture; Spinal cord

Indexed keywords

EID: 80055112090     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1773     Document Type: Article

References (6)

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2. Fabre, P., Shimogori, T., Charron, F. Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh Receptor Boc. Journal of Neuroscience, 30, 266-275 (2010).
3. Helms, A.W., Johnson, J.E. Progenitors of dorsal commissural interneurons are defined by MATH1 expression. Development, 125, 919-28 (1998).
4. Kaech, S., Banker, G. Culturing hippocampal neurons. Nature Protocols, 1, 2406-15 (2006).
5. Okada, A., Charron, F., Morin, S., Shin, D.S., Wong, K., Fabre, P.J., Tessier-Lavigne M., McConnell, S.K. Boc is a receptor for sonic hedgehog in the guidance of commissural axons. Nature, 444, 369-73s (2006).
6. Yam, P.T., Langlois, S.D., Morin, S., Charron, F. Sonic hedgehog guides axons through a noncanonical, Src-family-kinase-dependent signaling pathway. Neuron, 62, 349-62 (2009).